SANT proteins modulate gene expression by coordinating histone H3KAc and Khib levels and regulate plant heat tolerance

Zhou, Xishi1; Fan, Yujin1,2,3; Zhu, Xiying1,2,3; Zhao, Ruihua1; He, Junna4; Li, Pengfeng1; Shang, Shengping1; Goodrich, Justin5; Zhu, Jian-Kang6,7,8; Zhang, Cui-Jun1

2024-06-01

10.1093/plphys/kiae348

PLANT PHYSIOLOGY   影响因子和分区

0032-0889

1532-2548

["Histone post-translational modifications (PTMs), such as acetylation and recently identified lysine 2-hydroxyisobutyrylation (Khib), act as active epigenomic marks in plants. SANT domain-containing proteins SANT1, SANT2, SANT3, and SANT4 (SANT1/2/3/4), derived from PIF/Harbinger transposases, form a complex with HISTONE DEACETYLASE 6 (HDA6) to regulate gene expression via histone deacetylation. However, whether SANT1/2/3/4 coordinates different types of PTMs to regulate transcription and mediate responses to specific stresses in plants remains unclear. Here, in addition to modulating histone deacetylation, we found that SANT1/2/3/4 proteins acted like HDA6 or HDA9 in regulating the removal of histone Khib in Arabidopsis (Arabidopsis thaliana). Histone H3 lysine acetylation (H3KAc) and histone Khib were coordinated by SANT1/2/3/4 to regulate gene expression, with H3KAc playing a predominant role and Khib acting complementarily to H3KAc. SANT1/2/3/4 mutation significantly increased the expression of heat-inducible genes with concurrent change of H3KAc levels under normal and heat stress conditions, resulting in enhanced thermotolerance. This study revealed the critical roles of Harbinger transposon-derived SANT domain-containing proteins in transcriptional regulation by coordinating different types of histone PTMs and in the regulation of plant thermotolerance by mediating histone acetylation modification.","Proteins containing a histone-tail-binding module coordinate histone lysine acetylation and 2-hydroxyisobutyrylation and play critical roles in transcriptional regulation and plant thermotolerance."]

SCI

英语

其他

National Natural Science Foundation of China[32170610] ; Guang Dong Basic and Applied Basic Research Foundation[2022A1515111040]

Plant Sciences

Plant Sciences

WOS:001264228100001

OXFORD UNIV PRESS INC

PLANT & ANIMAL SCIENCE

Web of Science

1.Chinese Acad Agr Sci, Shenzhen Branch, Guangdong Lab Lingnan Modern Agr, Key Lab Synth Biol,Minist Agr & Rural Affairs,Agr, Shenzhen 518120, Peoples R China
2.Henan Univ, Sch Life Sci, Kaifeng 475004, Peoples R China
3.Henan Univ, Shenzhen Res Inst, Shenzhen 518000, Peoples R China
4.China Agr Univ, Coll Hort, Beijing 100193, Peoples R China
5.Univ Edinburgh, Inst Mol Plant Sci, Sch Biol Sci, Daniel Rutherford Bldg,Max Born Crescent, Edinburgh EH9 3BF, Scotland
6.Southern Univ Sci & Technol, Inst Adv Biotechnol, Shenzhen 518000, Peoples R China
7.Southern Univ Sci & Technol, Sch Life Sci, Shenzhen 518000, Peoples R China
8.Chinese Acad Agr Sci, Ctr Adv Bioind Technol, Beijing 100000, Peoples R China

Zhou, Xishi,Fan, Yujin,Zhu, Xiying,et al. SANT proteins modulate gene expression by coordinating histone H3KAc and Khib levels and regulate plant heat tolerance[J]. PLANT PHYSIOLOGY,2024.

Zhou, Xishi.,Fan, Yujin.,Zhu, Xiying.,Zhao, Ruihua.,He, Junna.,...&Zhang, Cui-Jun.(2024).SANT proteins modulate gene expression by coordinating histone H3KAc and Khib levels and regulate plant heat tolerance.PLANT PHYSIOLOGY.

Zhou, Xishi,et al."SANT proteins modulate gene expression by coordinating histone H3KAc and Khib levels and regulate plant heat tolerance".PLANT PHYSIOLOGY (2024).