DLM-GelMA/tumor slice sandwich structured tumor on a chip for drug efficacy testing

Hu, Wenqi1,2,3,4; Bei, Ho-Pan5; Jiang, Hongwei6,7; Wu, Di1,2,3,4; Yu, Xiaorui1,2,3,4; Zhou, Xintong5; Sun, Qiuwan8; Lu, Qinrui8; Du, Qijun1,2,3,4; Wang, Liangwen9; Luo, Zhi10; Wu, Guohua6,7; Zhao, Xin5,11; Wang, Shuqi1,2,3,4

2024-06-01

10.1039/d4lc00278d

LAB ON A CHIP   影响因子和分区

1473-0197

1473-0189

["The in vitro recapitulation of tumor microenvironment is of great interest to preclinical screening of drugs. Compared with culture of cell lines, tumor organ slices can better preserve the complex tumor architecture and phenotypic activity of native cells, but are limited by their exposure to fluid shear and gradual degradation under perfusion culture. Here, we established a decellularized liver matrix (DLM)-GelMA \"sandwich\" structure and a perfusion-based microfluidic platform to support long-term culture of tumor slices with excellent structural integrity and cell viability over 7 days. The DLM-GelMA was able to secrete cytokines and growth factors while providing shear protection to the tumor slice via the sandwich structure, leading to the preservation of the tumor microenvironment where immune cells (CD3, CD8, CD68), tumor-associated fibroblasts (alpha-SMA), and extracellular matrix components (collagen I, fibronectin) were well maintained. Furthermore, this chip presented anti-tumor efficacy at cisplatin (20 mu M) on tumor patients, demonstrating our platform's efficacy to design patient-specific treatment regimens. Taken together, the successful development of this DLM-GelMA sandwich structure on the chip could faithfully reflect the tumor microenvironment and immune response, accelerating the screening process of drug molecules and providing insights for practical medicine.","DLM-GelMA sandwich structure tumor slice-on-a-chip could faithfully reflect the tumor microenvironment and immune response for drug efficacy screening."]

SCI ; EI

英语

其他

National Key Research and Development Program of China["2022YFB3804700","2022YFA1105200"] ; West China Hospital, Sichuan University[ZYYC21010] ; Organ-on-a-Chip Innovation Base, Central Government-Guided Special Project for Local Scientific and Technological Development in Sichuan Province[2023ZYD0166] ; Chengdu City "Unveiling and Commanding" Science and Technology Project[2024-JB00-00018-GX] ; Collaborative Research Fund with World-leading Research Groups Hong Kong Polytechnic University[P0039523]

Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation

Biochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation

WOS:001260045000001

ROYAL SOC CHEMISTRY

CHEMISTRY

Web of Science

1.Sichuan Univ, West China Hosp, Prov Clin Res Ctr Resp Dis, Dept Resp & Crit Care Med, Chengdu 610065, Peoples R China
2.Sichuan Univ, Natl Engn Res Ctr Biomat, Chengdu 610065, Peoples R China
3.Sichuan Univ, Coll Biomed Engn, Chengdu 610065, Peoples R China
4.Tianfu Jincheng Lab, City Future Med, Chengdu 641400, Peoples R China
5.Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Hung Hom, Hong Kong 999077, Peoples R China
6.Henan Univ Sci & Technol, Affiliated Hosp 1, Endocrinol & Metab Ctr, Henan Key Lab Rare Dis, Luoyang 471003, Peoples R China
7.Henan Univ Sci & Technol, Coll Clin Med, Luoyang 471003, Peoples R China
8.Sichuan Diya BioTechnol Grp Co, Chengdu 641400, Peoples R China
9.Fudan Univ, Zhongshan Hosp, Dept Intervent Radiol, Shanghai 200032, Peoples R China
10.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
11.Hong Kong Polytech Univ, Res Inst Intelligent Wearable Syst, Hung Hom, Kowloon, Hong Kong 999077, Peoples R China

Hu, Wenqi,Bei, Ho-Pan,Jiang, Hongwei,et al. DLM-GelMA/tumor slice sandwich structured tumor on a chip for drug efficacy testing[J]. LAB ON A CHIP,2024.

Hu, Wenqi.,Bei, Ho-Pan.,Jiang, Hongwei.,Wu, Di.,Yu, Xiaorui.,...&Wang, Shuqi.(2024).DLM-GelMA/tumor slice sandwich structured tumor on a chip for drug efficacy testing.LAB ON A CHIP.

Hu, Wenqi,et al."DLM-GelMA/tumor slice sandwich structured tumor on a chip for drug efficacy testing".LAB ON A CHIP (2024).