Skilled reaching test for shoulder function assessment in a rat model of rotator cuff tear: a pilot study

Liu, Yang1,2,3,4; Fu, Sai-Chuen2,5; Yao, Shi-Yi2; Yung, Patrick Shu-Hang2

2024-06-28

10.1186/s12891-024-07624-6

BMC MUSCULOSKELETAL DISORDERS   影响因子和分区

1471-2474

BackgroundFunctional assessments are crucial to evaluate treatment outcomes in clinical and animal studies on rotator cuff injuries. While gait analysis is commonly used to assess animal models of rotator cuff tears, it is less relevant for human patients as the human shoulder is typically assessed in a non-weight-bearing condition. The present study introduces the skilled reaching test as a shoulder functional assessment tool for rats, which allows for evaluation without weight bearing.MethodsIn the control group, 8 male Sprague-Dawley rats received rotator cuff tear surgery without repair. In the rotator cuff repair group, 20 rats received rotator cuff repair at 4 weeks post rotator cuff tear. For the skilled reaching test, rats were trained to extend their forelimbs to fetch food pellets, and the number of trials, number of attempts and the success rate were recorded. The gait analysis and skilled reaching test were performed at baseline, 4 weeks post-tear, 1, 2, 4, and 8 weeks post-repair. The repeated measures analysis of variance was used to evaluate the effects of time on the shoulder function. The significance level was set at 0.05.ResultsThe skilled reaching test required 216 h to conduct, while the gait analysis took 44 h. In the rotator cuff repair group, gait performance significantly deteriorated at 1 week post-repair and restored to 4 weeks post-tear levels at 4 weeks post-repair. Regarding the skilled reaching test, the number of attempts, number of trials and the success rate decreased at 1 week post-repair. Subsequently, there was a brief rebound in performance observed at 2 weeks post-repair, followed by a continued decline in the number of attempts and trials. By 8 weeks post-repair, only the success rate had restored to levels similar to those observed at 4 weeks post-tear.ConclusionThe skilled reaching test can detect functional deficiencies following rotator cuff tear and repair, while it requires high time and labour costs.

Skilled reaching test Gait analysis Rotator cuff repair Shoulder function Animal model

SCI

英语

其他

Orthopedics ; Rheumatology

Orthopedics ; Rheumatology

WOS:001258649800002

BMC

CLINICAL MEDICINE

Web of Science

1.Shenzhen Peoples Hosp, Dept Bone & Joint Surg, Shenzhen 518020, Guangdong, Peoples R China
2.Chinese Univ Hong Kong, Prince Wales Hosp, Fac Med, Dept Orthopaed & Traumatol,Shatin, Lui Che Woo Clin Sci Bldg,5-F Room, Hong Kong 74029, Peoples R China
3.Jinan Univ, Clin Med Coll 2, Shenzhen, Guangdong, Peoples R China
4.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen, Guangdong, Peoples R China
5.Chinese Univ Hong Kong, Inst Innovat Med, Hong Kong, Peoples R China

Liu, Yang,Fu, Sai-Chuen,Yao, Shi-Yi,et al. Skilled reaching test for shoulder function assessment in a rat model of rotator cuff tear: a pilot study[J]. BMC MUSCULOSKELETAL DISORDERS,2024,25(1).

Liu, Yang,Fu, Sai-Chuen,Yao, Shi-Yi,&Yung, Patrick Shu-Hang.(2024).Skilled reaching test for shoulder function assessment in a rat model of rotator cuff tear: a pilot study.BMC MUSCULOSKELETAL DISORDERS,25(1).

Liu, Yang,et al."Skilled reaching test for shoulder function assessment in a rat model of rotator cuff tear: a pilot study".BMC MUSCULOSKELETAL DISORDERS 25.1(2024).