Characterization and Abundance of Plasmid-Dependent Alphatectivirus Bacteriophages

Parra, Boris1,2,3; Lutz, Veronika T.4; Brondsted, Lone4; Carmona, Javiera L.1; Palomo, Alejandro5; Nesme, Joseph6; Le, Vuong Van Hung6; Smets, Barth F.1; Dechesne, Arnaud1,7

2024-12-01

10.1007/s00248-024-02401-3

MICROBIAL ECOLOGY   影响因子和分区

0095-3628

1432-184X

Antimicrobial resistance (AMR) is a major public health threat, exacerbated by the ability of bacteria to rapidly disseminate antimicrobial resistance genes (ARG). Since conjugative plasmids of the incompatibility group P (IncP) are ubiquitous mobile genetic elements that often carry ARG and are broad-host-range, they are important targets to prevent the dissemination of AMR. Plasmid-dependent phages infect plasmid-carrying bacteria by recognizing components of the conjugative secretion system as receptors. We sought to isolate plasmid-dependent phages from wastewater using an avirulent strain of Salmonella enterica carrying the conjugative IncP plasmid pKJK5. Irrespective of the site, we only obtained bacteriophages belonging to the genus Alphatectivirus. Eleven isolates were sequenced, their genomes analyzed, and their host range established using S. enterica, Escherichia coli, and Pseudomonas putida carrying diverse conjugative plasmids. We confirmed that Alphatectivirus are abundant in domestic and hospital wastewater using culture-dependent and culture-independent approaches. However, these results are not consistent with their low or undetectable occurrence in metagenomes. Therefore, overall, our results emphasize the importance of performing phage isolation to uncover diversity, especially considering the potential of plasmid-dependent phages to reduce the spread of ARG carried by conjugative plasmids, and to help combat the AMR crisis.

Bacteria Bacteriophages Virus Plasmid Horizontal gene transfer Conjugation Antimicrobial resistance

SCI

英语

其他

European Union[101026675] ; VILLUM FONDEN[23046] ; VRID Postdoctorado from Vicerrectoria de Investigacion y Desarrollo, Universidad de Concepcion[CO 002200000279]

Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology

Ecology ; Marine & Freshwater Biology ; Microbiology

WOS:001258082800001

SPRINGER

ENVIRONMENT/ECOLOGY

Web of Science

1.Tech Univ Denmark, Dept Environm Engn & Resource Engn, Lyngby, Denmark
2.Univ Concepcion, Fac Ciencias Biol, Dept Microbiol, Lab Invest Agentes Antibacterianos LIAA, Concepcion, Chile
3.Univ Las Amer, Fac Med Vet & Agron, Inst Ciencias Nat, Concepcion, Chile
4.Univ Copenhagen, Dept Vet Anim Sci, Copenhagen, Denmark
5.Southern Univ Sci & Technol, Sch Environm Sci & Engn, Shenzhen, Peoples R China
6.Univ Copenhagen, Dept Biol, Sect Microbiol, Copenhagen, Denmark
7.Tech Univ Denmark, Dept Biotechnol & Biomed, Soltofs Plads,Bldg 221, DK-2800 Lyngby, Denmark

Parra, Boris,Lutz, Veronika T.,Brondsted, Lone,et al. Characterization and Abundance of Plasmid-Dependent Alphatectivirus Bacteriophages[J]. MICROBIAL ECOLOGY,2024,87(1).

Parra, Boris.,Lutz, Veronika T..,Brondsted, Lone.,Carmona, Javiera L..,Palomo, Alejandro.,...&Dechesne, Arnaud.(2024).Characterization and Abundance of Plasmid-Dependent Alphatectivirus Bacteriophages.MICROBIAL ECOLOGY,87(1).

Parra, Boris,et al."Characterization and Abundance of Plasmid-Dependent Alphatectivirus Bacteriophages".MICROBIAL ECOLOGY 87.1(2024).