Comparative time-dependent proteomics reveal the tolerance of cancer cells to magnetic iron oxide nanoparticles

Liu, Yanqing1; Meng, Yuqing1; Zhu, Yongping1; Gu, Liwei1; Ma, Ang1; Liu, Rui4; Liu, Dandan1; Shen, Shengnan1; Zhang, Shujie1; Xu, Chengchao1; Zhang, Junzhe1; Wang, Jigang1,2,3

2024-06-25

10.1093/rb/rbae065

REGENERATIVE BIOMATERIALS   影响因子和分区

2056-3418

2056-3426

Cancer is one of the most challenging diseases in the world. Recently, iron oxide nanoparticles (IONPs) are emerging materials with rapid development and high application value, and have shown great potential on tumor therapy due to their unique magnetic and biocompatible properties. However, some data hint us that IONPs were toxic to normal cells and vital organs. Thus, more data on biosafety evaluation is urgently needed. In this study, we compared the effects of silicon-coated IONPs (Si-IONPs) on two cell types: the tumor cells (Hela) and the normal cells (HEK293T, as 293 T for short), compared differences of protein composition, allocation and physical characteristics between these two cells. The major findings of our study pointed out that 293 T cells death occurred more significant than that of Hela cells after Si-IONPs treatment, and the rate and content of endocytosis of Si-IONPs in 293 T cells was more prominent than in Hela cells. Our results also showed Si-IONPs significant promoted the production of reactive oxygen species and disturbed pathways related to oxidative stress, iron homeostasis, apoptosis and ferroptosis in both two types of cells, however, Hela cells recovered from these disturbances more easily than 293 T. In conclusion, compared with Hela cells, IONPs are more likely to induce 293 T cells death and Hela cells have their own unique mechanisms to defense invaders, reminding scientists that future in vivo and in vitro studies of nanoparticles need to be cautious, and more safety data are needed for further clinical treatment.

iron oxide nanoparticles tumor therapy biosafety evaluation cell death

SCI ; EI

英语

通讯

Fundamental Research Funds for the National Natural Science Foundation["82204672","82204322","82104480"] ; China Academy of Chinese Medical Sciences Innovation Fund[CI2023E002] ; Central Public Welfare Research Institutes["ZZ16-ND-10-11","ZZ13-YQ-103","ZZ13-YQ-105","ZZ14-YQ-055","ZZ14-YQ-058","ZZ15-YQ-065","ZZ15- YQ-062","ZZ15-YQ-063","ZZ16-ND-10-05","ZZ16-ND-10-15","ZZ16- ND-10-17","ZZ16-ND-10-25","ZZ17-ND-10-11"]

Materials Science

Materials Science, Biomaterials

WOS:001253842600001

OXFORD UNIV PRESS

Web of Science

1.Inst Chinese Mat Med, China Acad Chinese Med Sci, Artemisinin Res Ctr, State Key Lab Qual Ensurance & Sustainable Use Dao, Beijing 100700, Peoples R China
2.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Dept Nephrol,Shenzhen key Lab Kidney Dis, Shenzhen 518020, Peoples R China
3.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Shenzhen Clin Res Ctr Geriatr, Shenzhen 518020, Peoples R China
4.Henan Agr Univ, Coll Anim Sci & Technol, Zhengzhou 450002, Peoples R China

Liu, Yanqing,Meng, Yuqing,Zhu, Yongping,et al. Comparative time-dependent proteomics reveal the tolerance of cancer cells to magnetic iron oxide nanoparticles[J]. REGENERATIVE BIOMATERIALS,2024,11.

Liu, Yanqing.,Meng, Yuqing.,Zhu, Yongping.,Gu, Liwei.,Ma, Ang.,...&Wang, Jigang.(2024).Comparative time-dependent proteomics reveal the tolerance of cancer cells to magnetic iron oxide nanoparticles.REGENERATIVE BIOMATERIALS,11.

Liu, Yanqing,et al."Comparative time-dependent proteomics reveal the tolerance of cancer cells to magnetic iron oxide nanoparticles".REGENERATIVE BIOMATERIALS 11(2024).