Proteomic insights into composition-dependent effects of microplastics on freshwater microalgae Chlamydomonas reinhardtii

Xu, Liting1,2; Chu, Zheng1,2; Li, Xiaoxue1,2,6; Feng, Chenran1,2; Zhang, Ying1,2; Wang, Chen1,2; Zhang, Junzhe1,2; Xu, Chengchao1,2; Wang, Jigang1,2,3,4,5; Tang, Huan1,2

2024-06-01

10.1039/d4en00300d

ENVIRONMENTAL SCIENCE-NANO   影响因子和分区

2051-8153

2051-8161

The widespread presence of microplastics (MPs) in freshwater systems has raised concerns about their potential ecotoxicity to aquatic organisms. In this study, we evaluated the effects of four MPs with different compositions, namely polyethylene (PE), polystyrene (PS), polyvinyl chloride (PVC) and polypropylene (PP), on freshwater microalgae Chlamydomonas reinhardtii. PS and PVC MPs caused greater growth inhibition and stronger oxidative stress responses than PP and PE MPs. Proteomics analysis was employed to explore the mechanical understanding of the composition-dependent toxicity of MPs to microalgae. Proteins involved in photosynthesis processes were identified as contributors to the diverse responses of microalgae to differently composed MPs. The photosynthesis activity of algae, including the pigment content and photoprotective response, was determined to reflect the distinct effect of the four MPs. The indicated down-regulated expression of photosynthetic proteins by proteomics analysis was further confirmed using a western blot assay, with PVC and PS showing greater impacts on their expression reduction. Our findings not only show the composition-dependent effect of MPs on microalgae but also provide important insights into the molecular mechanism of MPs' toxicity to natural phytoplankton species.

SCI ; EI

英语

通讯

CACMS Innovation Fund["CI2023E002","CI2021A05101","CI2021A05104"] ; National Key Research and Development Program of China["2020YFA0908000","2022YFC2303600"] ; Innovation Team and Talents Cultivation Program of the National Administration of Traditional Chinese Medicine[ZYYCXTD-C-202002] ; National Natural Science Foundation of China[32201177] ; Fundamental Research Funds for the Central Public Welfare Research Institutes["ZZ14-YQ-061","ZZ16-ND-10-24","ZZ17-ND-10-15","ZZ14-FL-002"] ; China Postdoctoral Science Fund[2022M711535]

Chemistry ; Environmental Sciences & Ecology ; Science & Technology - Other Topics

Chemistry, Multidisciplinary ; Environmental Sciences ; Nanoscience & Nanotechnology

WOS:001254861700001

ROYAL SOC CHEMISTRY

Web of Science

1.China Acad Chinese Med Sci, State Key Lab Qual Ensurance & Sustainable Use Dao, Artemisinin Res Ctr, Beijing 100700, Peoples R China
2.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China
3.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Shenzhen Clin Res Ctr Geriatr, Dept Nephrol, Shenzhen 518020, Guangdong, Peoples R China
4.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China
5.Henan Univ, Sch Pharm, State Key Lab Antiviral Drugs, Kaifeng 475004, Peoples R China
6.Tianjin Acad Agr Sci, Inst Agr Prod Preservat & Proc Technol, Natl Engn Technol Res Ctr Preservat Agr Prod, Tianjin 300384, Peoples R China

Xu, Liting,Chu, Zheng,Li, Xiaoxue,et al. Proteomic insights into composition-dependent effects of microplastics on freshwater microalgae Chlamydomonas reinhardtii[J]. ENVIRONMENTAL SCIENCE-NANO,2024.

Xu, Liting.,Chu, Zheng.,Li, Xiaoxue.,Feng, Chenran.,Zhang, Ying.,...&Tang, Huan.(2024).Proteomic insights into composition-dependent effects of microplastics on freshwater microalgae Chlamydomonas reinhardtii.ENVIRONMENTAL SCIENCE-NANO.

Xu, Liting,et al."Proteomic insights into composition-dependent effects of microplastics on freshwater microalgae Chlamydomonas reinhardtii".ENVIRONMENTAL SCIENCE-NANO (2024).