The Olfactory Working Memory Capacity Paradigm

Jiang, Lixin1,2,3,4; Huang, Gengdi5,6; Yu, Xin1,2,3,4

2024-06-01

10.1002/cpz1.1072

CURRENT PROTOCOLS   影响因子和分区

2691-1299

Working memory capacity (WMC), a crucial component of working memory (WM), has consistently drawn the attention of researchers. Exploring the underlying neurobiological mechanisms behind it is currently a prominent focus in the field of neuroscience. Previously, we developed a novel behavioral paradigm for rodents called the olfactory working memory capacity (OWMC) paradigm, which serves as an effective tool for quantifying the WMC of rodents. The OWMC task comprises five phases: context adaptation, digging training, rule-learning for nonmatching to a single sample odor (NMSS), rule-learning for nonmatching to multiple sample odors (NMMS), and capacity testing. In the first phase, mice are handled to reduce stress and acclimate to the training cage. The second phase involves training mice to dig in a bowl of unscented sawdust to locate a piece of cheese. In the third phase, mice are trained to locate the cheese pellet in a bowl with a noveal odor. The fourth phase requires mice to distinguish the novel odor among multiple scented bowls to locate the cheese pellet. Finally, in the fifth phase, mice undergo several WMC tests until they achieve a stable level of performance. In this protocol paper, we will provide detailed instructions on how to implement the behavioral paradigm. (c) 2024 Wiley Periodicals LLC.Basic Protocol 1: Context adaptationBasic Protocol 2: Digging trainingBasic Protocol 3: Rule-learning for NMSSBasic Protocol 4: Rule-learning for NMMSBasic Protocol 5: Capacity testing

mice odor working memory capacity

ESCI

英语

其他

National Natural Science Foundation of China[82101492] ; Shenzhen Science and Technology Program[RCBS20221008093252091]

Biochemistry & Molecular Biology

Biochemical Research Methods

WOS:001248153400001

WILEY

Web of Science

1.Peking Univ, Hosp 6, Inst Mental Hlth, Beijing, Peoples R China
2.Peking Univ, Natl Clin Res Ctr Mental Disorders, Beijing, Peoples R China
3.Peking Univ, NHC Key Lab Mental Hlth, Beijing, Peoples R China
4.Beijing Municipal Key Lab Translat Res Diag & Trea, Beijing, Peoples R China
5.Shenzhen Clin Res Ctr Mental Disorders, Shenzhen Mental Hlth Ctr, Dept Addict Med, Shenzhen, Peoples R China
6.Southern Univ Sci & Technol, Affiliated Mental Hlth Ctr, Shenzhen, Peoples R China

Jiang, Lixin,Huang, Gengdi,Yu, Xin. The Olfactory Working Memory Capacity Paradigm[J]. CURRENT PROTOCOLS,2024,4(6).

Jiang, Lixin,Huang, Gengdi,&Yu, Xin.(2024).The Olfactory Working Memory Capacity Paradigm.CURRENT PROTOCOLS,4(6).

Jiang, Lixin,et al."The Olfactory Working Memory Capacity Paradigm".CURRENT PROTOCOLS 4.6(2024).