南方科技大学知识苑(SUSTech KC): YjgA plays dual roles in enhancing PTC maturation

题名	YjgA plays dual roles in enhancing PTC maturation
作者	Du, Mengtan 1,2; Deng, Chenke 1; Yu, Ting1,2 ; Zhou, Qixin1,2 ; Zeng, Fuxing1,2
通讯作者	Zeng, Fuxing
发表日期	2024-06-01
DOI	10.1093/nar/gkae469
发表期刊	NUCLEIC ACIDS RESEARCH 影响因子和分区
ISSN	0305-1048
EISSN	1362-4962
摘要	["Ribosome biogenesis is a highly regulated cellular process that involves the control of numerous assembly factors. The small protein YjgA has been reported to play a role in the late stages of 50S assembly. However, the precise molecular mechanism underlying its function remains unclear. In this study, cryo-electron microscopy (cryo-EM) structures revealed that depletion of YjgA or its N-terminal loop in Escherichia coli both lead to the accumulation of immature 50S particles with structural abnormalities mainly in peptidyl transferase center (PTC) and H68/69 region. CryoDRGN analysis uncovered 8 and 6 distinct conformations of pre50S for Delta yjgA and YjgA-Delta Nloop, respectively. These conformations highlighted the role of the N-terminal loop of YjgA in integrating uL16 and stabilizing H89 in PTC, which was further verified by the pull-down assays of YjgA and its mutants with uL16. Together with the function of undocking H68 through the binding of its C-terminal CTLH-like domain to the base of the L1 stalk, YjgA facilitates the maturation of PTC. This study identified critical domains of YjgA contributing to 50S assembly efficiency, providing a comprehensive understanding of the dual roles of YjgA in accelerating ribosome biogenesis and expanding our knowledge of the intricate processes governing cellular protein synthesis.","Graphical Abstract"]
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	National Natural Science Foundation of China[Optima XPN-100]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:001241817700001
出版者	OXFORD UNIV PRESS
ESI学科分类	BIOLOGY & BIOCHEMISTRY
来源库	Web of Science
引用统计
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/788119
专题	生命科学学院南方科技大学生命科学学院_系统生物学系
作者单位	1.Southern Univ Sci & Technol, Sch Life Sci, Dept Syst Biol, 1088 Xueyuan Ave, Shenzhen 518055, Peoples R China 2.Southern Univ Sci & Technol, Inst Biol Electron Microscopy, 1088 Xueyuan Ave, Shenzhen 518055, Guangdong, Peoples R China
第一作者单位	生命科学学院; 南方科技大学
通讯作者单位	生命科学学院; 南方科技大学
第一作者的第一单位	生命科学学院
推荐引用方式 GB/T 7714	Du, Mengtan,Deng, Chenke,Yu, Ting,et al. YjgA plays dual roles in enhancing PTC maturation[J]. NUCLEIC ACIDS RESEARCH,2024.
APA	Du, Mengtan,Deng, Chenke,Yu, Ting,Zhou, Qixin,&Zeng, Fuxing.(2024).YjgA plays dual roles in enhancing PTC maturation.NUCLEIC ACIDS RESEARCH.
MLA	Du, Mengtan,et al."YjgA plays dual roles in enhancing PTC maturation".NUCLEIC ACIDS RESEARCH (2024).