Transgenerational increases in DNA methylation in Arabidopsis plants defective in active DNA demethylation

Tang, Kai1; Zhu, Xiaohong2,3; Xie, Shaojun4; Lang, Zhaobo5,6; Zhu, Jian-Kang5,6

2024-05-28

10.1073/pnas.2320468121

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   影响因子和分区

0027-8424

1091-6490

Spontaneous gain or loss of DNA methylation occurs in plant and animal genomes, and DNA methylation changes can lead to meiotically stable epialleles that generate heritable phenotypic diversity. However, it is unclear whether transgenerational epigenetic stability may be regulated by any cellular factors. Here, we examined spontaneously occurring variations in DNA methylation in wild - type and ros1 mutant Arabidopsis plants that were propagated for ten generations from single - seed descent. We found that the ros1 mutant, which is defective in active DNA demethylation, showed an increased transgenerational epimutation rate. The ros1 mutation led to more spontaneously gained methylation than lost methylation at individual cytosines, compared to the wild type which had similar numbers of spontaneously gained and lost methylation cytosines. Consistently, transgenerational differentially methylated regions were also biased toward hypermethylation in the ros1 mutant. Our results reveal a genetic contribution of the ROS1 DNA demethylase to transgenerational epigenetic stability and suggest that ROS1 may have an unexpected surveillance function in preventing transgenerational DNA methylation increases.

DNA methylation transgenerational DNA methylation ros1 mutation

SCI

英语

通讯

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:001237741800006

NATL ACAD SCIENCES

BIOLOGY & BIOCHEMISTRY ; CLINICAL MEDICINE ; MULTIDISCIPLINARY ; PLANT & ANIMAL SCIENCE ; ENVIRONMENT/ECOLOGY ; SOCIAL SCIENCES, GENERAL ; MICROBIOLOGY ; ECONOMICS BUSINESS ; IMMUNOLOGY ; MATERIALS SCIENCE ; MATHEMATICS ; SPACE SCIENCE ; MOLECULAR BIOLOGY & GENETICS ; PHARMACOLOGY & TOXICOLOGY ; CHEMISTRY ; PSYCHIATRY/PSYCHOLOGY ; NEUROSCIENCE & BEHAVIOR ; PHYSICS ; GEOSCIENCES ; AGRICULTURAL SCIENCES ; ENGINEERING

Web of Science

1.Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA
2.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng 475004, Peoples R China
3.Henan Univ, State Key Lab Cotton Biol, Kaifeng 475004, Peoples R China
4.Purdue Univ, Bioinformat Core, W Lafayette, IN 47907 USA
5.Southern Univ Sci & Technol, Inst Adv Biotechnol, Shenzhen 518055, Peoples R China
6.Southern Univ Sci & Technol, Sch Med, Shenzhen 518055, Peoples R China

Tang, Kai,Zhu, Xiaohong,Xie, Shaojun,et al. Transgenerational increases in DNA methylation in Arabidopsis plants defective in active DNA demethylation[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2024,121(22).

Tang, Kai,Zhu, Xiaohong,Xie, Shaojun,Lang, Zhaobo,&Zhu, Jian-Kang.(2024).Transgenerational increases in DNA methylation in Arabidopsis plants defective in active DNA demethylation.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,121(22).

Tang, Kai,et al."Transgenerational increases in DNA methylation in Arabidopsis plants defective in active DNA demethylation".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 121.22(2024).