Electrolyte-gated amorphous IGZO transistors with extended gates for prostate-specific antigen detection

Yin, Xuemei1; Ji, Xingqi1; Liu, Wenlong1; Li, Xiaoqian1; Wang, Mingyang1; Xin, Qian1,2; Zhang, Jiawei1; Yan, Zhuocheng1; Song, Aimin3,4

2024-06-25

10.1039/d4lc00247d

LAB ON A CHIP   影响因子和分区

1473-0197

1473-0189

The prostate-specific antigen (PSA) test is considered an important way for preoperative diagnosis and accurate screening of prostate cancer. Current antigen detection methods, including radioimmunoassay, enzyme-linked immunosorbent assay and microfluidic electrochemical detection, feature expensive equipment, long testing time and poor stability. Here, we propose a portable biosensor composed of electrolyte-gated amorphous indium gallium zinc oxide (a-IGZO) transistors with an extended gate, which can achieve real-time, instant PSA detection at a low operating voltage (<2 V) owing to the liquid-free ionic conductive elastomer (ICE) serving as the gate dielectric. The electric double layer (EDL) capacitance in ICE enhances the accumulation of carriers in the IGZO channel, leading to strong gate modulation, which enables the IGZO transistor to have a small subthreshold swing (<0.5 V dec(-1)) and a high on-state current (similar to 4 x 10(-4) A). The separate, biodegradable, and pluggable sensing pad, serving as an extended gate connected to the IGZO transistor, prevents contamination and depletion arising from direct contact with biomolecular buffers, enabling the IGZO transistor to maintain superior electronic performance for at least six months. The threshold voltage and channel current of the transistor exhibit excellent linear response to PSA molecule concentrations across five orders of magnitude ranging from 1 fg mL(-1) to 10 pg mL(-1), with a detection limit of 400 ag mL(-1) and a detection time of similar to 5.1 s. The fabricated biosensors offer a point-of-care system for antigen detection, attesting the feasibility of the electrolyte-gated transistors in clinical screening, healthcare diagnostics and biological management.

SCI ; EI

英语

通讯

National Key Research and Development Program of China["2022YFB3603900","2022YFA1405200"] ; National Key Research and Development Program of China[62074094] ; National Natural Science Foundation of China["IEC\\R2\\170155","NA170415"] ; Royal Society[2017GGX10111] ; Key Research and Development Program of Shandong Province["ZR2020ZD03","ZR2022ZD05","ZR2021QE148"]

Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation

Biochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation

WOS:001240966100001

ROYAL SOC CHEMISTRY

CHEMISTRY

Web of Science

1.Shandong Univ, Sch Integrated Circuits, Jinan 250100, Peoples R China
2.Shandong Univ, Inst Novel Semicond, State Key Lab Crystal Mat, Jinan 250100, Peoples R China
3.Southern Univ Sci & Technol, Inst Nanosci & Applicat, Shenzhen 518055, Peoples R China
4.Univ Manchester, Sch Elect & Elect Engn, Manchester M13 9PL, England

Yin, Xuemei,Ji, Xingqi,Liu, Wenlong,et al. Electrolyte-gated amorphous IGZO transistors with extended gates for prostate-specific antigen detection[J]. LAB ON A CHIP,2024,24(13).

Yin, Xuemei.,Ji, Xingqi.,Liu, Wenlong.,Li, Xiaoqian.,Wang, Mingyang.,...&Song, Aimin.(2024).Electrolyte-gated amorphous IGZO transistors with extended gates for prostate-specific antigen detection.LAB ON A CHIP,24(13).

Yin, Xuemei,et al."Electrolyte-gated amorphous IGZO transistors with extended gates for prostate-specific antigen detection".LAB ON A CHIP 24.13(2024).