Analysis of binding and authentic virus-neutralizing activities of immune sera induced by various monkeypox virus antigens

Song, Shuo1,2; Ren, Zuning3; Chen, Jiayin3; Li, Mengjun3; Jiang, Yushan3; Liu, Yingxia1,2; Zhang, Bao3; Lu, Hongzhou1,2; Zhao, Wei3,4; Shen, Chenguang3,4,5; Yang, Yang1,2

2024-06-01

10.1007/s12026-024-09499-0

IMMUNOLOGIC RESEARCH   影响因子和分区

0257-277X

1559-0755

Monkeypox cases continue to increase globally, and there is an urgent need to develop a highly effective vaccine against monkeypox. This study investigated the binding and authentic-virus neutralizing activities of sera from mice immunized with EEV (extracellularly enveloped viruses) antigens B6R and A35R, and IMV (intrinsic material viruses) antigens M1R, A29L, E8L, and H3L against monkeypox virus. The results showed that immunizations of A35R and E8L could only induce lower titers of binding antibodies, in contrast, immunization of M1R induced the highest titers of binding antibodies, while immunization of B6R, H3L, and A29L induced moderate titers of binding antibodies. For the live monkeypox virus neutralization assay, the results showed that immunization with two doses of EEV antigen B6R did not effectively induce humoral immune responses to neutralize monkeypox live virus, immunization with EEV-A35R only induced weak monkeypox-neutralizing antibodies. In contrast, the immunization of the four types of monkeypox virus IMV antigens can all induce neutralizing antibodies against authentic monkeypox virus, among them, A29L and H3L induced the highest neutralizing antibody titers. The results of this study provide important references for the selection of antigens in the development of the next generation of monkeypox vaccines.

Monkeypox Vaccine Authentic virus Binding antibody Neutralizing antibody

SCI

英语

第一 ; 通讯

Immunology

Immunology

WOS:001238135900001

SPRINGER

IMMUNOLOGY

Web of Science

1.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, Dept Infect Dis, Shenzhen, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 2, Shenzhen, Peoples R China
3.Southern Med Univ, Sch Publ Hlth, Guangdong Prov Key Lab Trop Dis Res, BSL 3 Lab Guangdong, Guangzhou, Peoples R China
4.Southern Med Univ, Key Lab Infect Dis Res South China, Minist Educ, Guangzhou, Peoples R China
5.Southern Med Univ, Zhujiang Hosp, Dept Lab Med, Guangzhou, Peoples R China

Song, Shuo,Ren, Zuning,Chen, Jiayin,et al. Analysis of binding and authentic virus-neutralizing activities of immune sera induced by various monkeypox virus antigens[J]. IMMUNOLOGIC RESEARCH,2024.

Song, Shuo.,Ren, Zuning.,Chen, Jiayin.,Li, Mengjun.,Jiang, Yushan.,...&Yang, Yang.(2024).Analysis of binding and authentic virus-neutralizing activities of immune sera induced by various monkeypox virus antigens.IMMUNOLOGIC RESEARCH.

Song, Shuo,et al."Analysis of binding and authentic virus-neutralizing activities of immune sera induced by various monkeypox virus antigens".IMMUNOLOGIC RESEARCH (2024).