南方科技大学知识苑(SUSTech KC): Enhanced osteogenic and ROS-scavenging MXene nanosheets incorporated gelatin-based nanocomposite hydrogels for critical-sized calvarial defect repair

题名	Enhanced osteogenic and ROS-scavenging MXene nanosheets incorporated gelatin-based nanocomposite hydrogels for critical-sized calvarial defect repair
作者	Zhao, Jin 1; Wang, Tiehua 2; Zhu, Yuanchao 1,3; Qin, Haotian 1; Qian, Junyu 1; Wang, Qichang 1; Zhang, Peng 1; Liu, Peng 1; Xiong, Ao 1; Li, Nan 4 ; Udduttula, Anjaneyulu 5; Ye, Sang-Ho 6; Wang, Deli 1; Zeng, Hui 1; Chen, Yingqi 1
通讯作者	Li, Nan; Zeng, Hui; Chen, Yingqi
发表日期	2024-06-01
DOI	10.1016/j.ijbiomac.2024.131914
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 影响因子和分区
ISSN	0141-8130
EISSN	1879-0003
卷号	269
摘要	The healing of critical-sized bone defects is a major challenge in the field of bone tissue engineering. Gelatinrelated hydrogels have emerged as a potential solution due to their desirable properties. However, their limited osteogenic, mechanical, and reactive oxygen species (ROS)-scavenging capabilities have hindered their clinical application. To overcome this issue, we developed a biofunctional gelatin-Mxene nanocomposite hydrogel. Firstly, we prepared two-dimensional (2D) Ti3C2 MXene nanosheets using a layer delamination method. Secondly, these nanosheets were incorporated into a transglutaminase (TG) enzyme-containing gallic acid-imbedded gelatin (GGA) pre-gel solution to create an injectable GGA-MXene (GM) nanocomposite hydrogel. The GM hydrogels exhibited superior compressive strength (44-75.6 kPa) and modulus (24-44.5 kPa) compared to the GGA hydrogels. Additionally, the GM hydrogel demonstrated the ability to scavenge reactive oxygen species (OH- and DPPH radicals), protecting MC3T3-E1 cells from oxidative stress. GM hydrogels were non-toxic to MC3T3-E1 cells, increased alkaline phosphatase secretion, calcium nodule formation, and upregulated osteogenic gene expressions (ALP, OCN, and RUNX2). The GM400 hydrogel was implanted in critical-sized calvarial defects in rats. Remarkably, it exhibited significant potential for promoting new bone formation. These findings indicated that GM hydrogel could be a viable candidate for future clinical applications in the treatment of critical-sized bone defects.
关键词	MXene nanosheets Gallic acid Gelatin nanocomposite hydrogel Bone defect ROS-scavenging Osteogenesis
相关链接	[来源记录]
收录类别	SCI ; EI
语种	英语
学校署名	通讯
资助项目	Natural Science Foundation of China["82202664","32000516","82172432"] ; Shenzhen Key Laboratory of Orthopaedic Diseases and Biomaterials Research[ZDSYS20220606100602005] ; Shenzhen Sustainable Development Project[KCXFZ20201221173411031] ; Guangdong Basic and Applied Basic Research Foundation["2023A1515012764","2021A1515220053","2022A1515220038"] ; Shenzhen Science and Technology Project["JCYJ20190809165805604","JCYJ20220818102815033","JCYJ20220531094214032"]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:001239036800001
出版者	ELSEVIER
ESI学科分类	BIOLOGY & BIOCHEMISTRY
来源库	Web of Science
引用统计	被引频次[WOS]：2
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/788252
专题	南方科技大学第一附属医院
作者单位	1.Peking Univ Shenzhen Hosp, Natl & Local Joint Engn Res Ctr Orthopaed Biomat, Dept Bone & Joint Surg, Shenzhen 518036, Peoples R China 2.Shenzhen New Frontier United Family Hosp, Internal Med, Shenzhen 518031, Peoples R China 3.Shenzhen Univ, Med Sch, Shenzhen 518055, Guangdong, Peoples R China 4.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1,Dept Stomatol,Clin Med Sch 2, Shenzhen 518020, Peoples R China 5.Vellore Inst Technol VIT, Ctr Biomat Cellular & Mol Theranost CBCMT, Vellore 632014, Tamil Nadu, India 6.Univ Pittsburgh, McGowan Inst Regenerat Med, Pittsburgh, PA 15219 USA
通讯作者单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Zhao, Jin,Wang, Tiehua,Zhu, Yuanchao,et al. Enhanced osteogenic and ROS-scavenging MXene nanosheets incorporated gelatin-based nanocomposite hydrogels for critical-sized calvarial defect repair[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,269.
APA	Zhao, Jin.,Wang, Tiehua.,Zhu, Yuanchao.,Qin, Haotian.,Qian, Junyu.,...&Chen, Yingqi.(2024).Enhanced osteogenic and ROS-scavenging MXene nanosheets incorporated gelatin-based nanocomposite hydrogels for critical-sized calvarial defect repair.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,269.
MLA	Zhao, Jin,et al."Enhanced osteogenic and ROS-scavenging MXene nanosheets incorporated gelatin-based nanocomposite hydrogels for critical-sized calvarial defect repair".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 269(2024).