Phagocytosis in Marine Coccolithophore Gephyrocapsa huxleyi: Comparison between Calcified and Non-Calcified Strains

Ye, Jiayang1,2,3; Wang, Ying1; Li, Qian1; Hussain, Sarfraz4; Chen, Songze5; Zhou, Xunying4; Hou, Shengwei2,3,4; Feng, Yuanyuan1,2,3

2024-05-01

10.3390/biology13050310

BIOLOGY-BASEL   影响因子和分区

2079-7737

Coccolithophores play a significant role in marine calcium carbonate production and carbon cycles, attributing to their unique feature of producing calcareous plates, coccoliths. Coccolithophores also possess a haplo-diplontic life cycle, presenting distinct morphology types and calcification states. However, differences in nutrient acquisition strategies and mixotrophic behaviors of the two life phases remain unclear. In this study, we conducted a series of phagocytosis experiments of calcified diploid and non-calcified haploid strains of coccolithophore Gephyrocapsa huxleyi under light and dark conditions. The phagocytosis capability of each strain was examined based on characteristic fluorescent signals from ingested beads using flow cytometry and fluorescence microscopy. The results show a significantly higher phagocytosis percentage on fluorescent beads in the bacterial prey surrogates of the non-calcified haploid Gephyrocapsa huxleyi strain, than the calcified diploid strain with or without light. In addition, the non-calcified diploid cells seemingly to presented a much higher phagocytosis percentage in darkness than under light. The differential phagocytosis capacities between the calcified diploid and non-calcified haploid Gephyrocapsa huxleyi strains indicate potential distinct nutritional strategies at different coccolithophore life and calcifying stages, which may further shed light on the potential strategies that coccolithophore possesses in unfavorable environments such as twilight zones and the expanding coccolithophore niches in the natural marine environment under the climate change scenario.

coccolithophore Gephyrocapsa huxleyi life stage haploid diploid calcification phagocytosis

SCI

英语

其他

Life Sciences & Biomedicine - Other Topics

Biology

WOS:001233551500001

MDPI

Web of Science

1.Shanghai Jiao Tong Univ, Sch Oceanog, Shanghai 200030, Peoples R China
2.Shanghai Jiao Tong Univ, Shanghai Key Lab Polar Life & Environm Sci, Shanghai 200030, Peoples R China
3.Shanghai Jiao Tong Univ, Key Lab Polar Ecosyst & Climate Change, Minist Educ, Shanghai 200030, Peoples R China
4.Southern Univ Sci & Technol, Dept Ocean Sci & Engn, Shenzhen 518055, Peoples R China
5.Shenzhen Ecol & Environm Monitoring Ctr Guangdong, Shenzhen 518049, Peoples R China

Ye, Jiayang,Wang, Ying,Li, Qian,et al. Phagocytosis in Marine Coccolithophore Gephyrocapsa huxleyi: Comparison between Calcified and Non-Calcified Strains[J]. BIOLOGY-BASEL,2024,13(5).

Ye, Jiayang.,Wang, Ying.,Li, Qian.,Hussain, Sarfraz.,Chen, Songze.,...&Feng, Yuanyuan.(2024).Phagocytosis in Marine Coccolithophore Gephyrocapsa huxleyi: Comparison between Calcified and Non-Calcified Strains.BIOLOGY-BASEL,13(5).

Ye, Jiayang,et al."Phagocytosis in Marine Coccolithophore Gephyrocapsa huxleyi: Comparison between Calcified and Non-Calcified Strains".BIOLOGY-BASEL 13.5(2024).