南方科技大学知识苑(SUSTech KC): Long Non-coding RNA PCED1B Antisense RNA 1 Promotes Cell Proliferation and Invasion in Hepatocellular Carcinoma by Regulating the MicroRNA-34a/CD44 Axis

题名	Long Non-coding RNA PCED1B Antisense RNA 1 Promotes Cell Proliferation and Invasion in Hepatocellular Carcinoma by Regulating the MicroRNA-34a/CD44 Axis
作者	Bi, Jian-gang 1,2; Li, Qi 1,2 ; Guo, Yu-sheng 1,2; Liu, Li-ping 1,2; Bao, Shi-yun 1,2; Xu, Ping 2,3
通讯作者	Xu, Ping
发表日期	2024-06-01
DOI	10.1007/s11596-023-2823-5
发表期刊	CURRENT MEDICAL SCIENCE 影响因子和分区
ISSN	2096-5230
EISSN	2523-899X
卷号	44 期号:3
摘要	ObjectiveThis study aimed to examine the role of long non-coding RNA PCED1B antisense RNA 1 (PCED1B-AS1) in the development of hepatocellular carcinoma (HCC).MethodsA total of 62 pairs of HCC tissues and adjacent non-tumor tissues were obtained from 62 HCC patients. The interactions of PCED1B-AS1 and microRNA-34a (miR-34a) were detected by dual luciferase activity assay and RNA pull-down assay. The RNA expression levels of PCED1B-AS1, miR-34a and CD44 were detected by RT-qPCR, and the protein expression level of CD44 was determined by Western blotting. The cell proliferation was detected by cell proliferation assay, and the cell invasion and migration by transwell invasion assay. The HCC tumor growth after PCED1B-AS1 was downregulated was determined by in vivo animal study.ResultsPCED1B-AS1 was highly expressed in HCC tissues, which was associated with poor survival of HCC patients. Furthermore, PCED1B-AS1 interacted with miR-34a in HCC cells, but they did not regulate the expression of each other. Additionally, PCED1B-AS1 increased the expression level of CD44, which was targeted by miR-34a. The cell proliferation and invasion assay revealed that miR-34a inhibited the proliferation and invasion of HCC in vitro, while CD44 exhibited the opposite effects. Furthermore, PCED1B-AS1 suppressed the role of miR-34a. Moreover, the knockdown of PCED1B-AS1 repressed the HCC tumor growth in nude mice in vivo.ConclusionPCED1B-AS1 may play an oncogenic role by regulating the miR-34a/CD44 axis in HCC.
关键词	long non-coding RNA PCED1B antisense RNA 1 (PCED1B-AS1) hepatocellular carcinoma microRNA-34a (miR-34a) CD44 proliferation invasion
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	通讯
WOS研究方向	Research & Experimental Medicine
WOS类目	Medicine, Research & Experimental
WOS记录号	WOS:001223897000003
出版者	SPRINGER
来源库	Web of Science
引用统计
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/788437
专题	南方科技大学第一附属医院
作者单位	1.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Hepatobiliary & Pancreat Surg, Shenzhen, Peoples R China 2.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Peoples R China 3.Jinan Univ, Clin Med Coll 2, Shenzhen Peoples Hosp, Dept Endocrinol, Shenzhen, Peoples R China
第一作者单位	南方科技大学第一附属医院
通讯作者单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Bi, Jian-gang,Li, Qi,Guo, Yu-sheng,et al. Long Non-coding RNA PCED1B Antisense RNA 1 Promotes Cell Proliferation and Invasion in Hepatocellular Carcinoma by Regulating the MicroRNA-34a/CD44 Axis[J]. CURRENT MEDICAL SCIENCE,2024,44(3).
APA	Bi, Jian-gang,Li, Qi,Guo, Yu-sheng,Liu, Li-ping,Bao, Shi-yun,&Xu, Ping.(2024).Long Non-coding RNA PCED1B Antisense RNA 1 Promotes Cell Proliferation and Invasion in Hepatocellular Carcinoma by Regulating the MicroRNA-34a/CD44 Axis.CURRENT MEDICAL SCIENCE,44(3).
MLA	Bi, Jian-gang,et al."Long Non-coding RNA PCED1B Antisense RNA 1 Promotes Cell Proliferation and Invasion in Hepatocellular Carcinoma by Regulating the MicroRNA-34a/CD44 Axis".CURRENT MEDICAL SCIENCE 44.3(2024).