Hyperbaric oxygen therapy enhances osteointegration of reimplanted cranial flap by regulating osteogenesis-angiogenesis coupling

Pan, Yonghao1,2; Li, Jiawei1,2; Wu, Jianqun3; Yang, Chengyu1,2; Wu, Siying1,2; Yang, Kunhua1; Yang, Xue4; Chen, Qian4,6; Fu, Guibing4,6; Liu, Chao1,2,5

2024-05-01

10.1002/jor.25875

JOURNAL OF ORTHOPAEDIC RESEARCH   影响因子和分区

0736-0266

1554-527X

Craniectomy is a lifesaving procedure to alleviate dangerously high intracranial pressure by removing a bone flap from the calvarium. However, the osteointegration of reimplanted bone flap with the existing bone tissue is still a clinical challenge. Hyperbaric oxygen (HBO) therapy has shown efficacy in promoting bone repair and could be a promising treatment for accelerating postoperative recovery. However, the specific cell types that are responsive to HBO treatment are not well understood. In this study, we created a murine model of craniectomy, with reimplantation of the cranial flap after 1 week. The effects of HBO treatment on bone formation and blood vessel formation around reimplanted bone were examined by micro-computed tomography, histological staining, and immunofluorescence staining. Single-cell RNA sequencing (scRNAseq) was utilized to identify key cell subtypes and signaling pathways after HBO treatment. We found that HBO treatment increased bone volume around reimplanted cranial flaps. HBO also increased the volume of Osterix-expressing cells and type H vessels. scRNAseq data showed more mature osteoblasts and endothelial cells, with higher expressions of adhesion and migration-related genes after HBO treatment. Cell-cell interaction analysis revealed a higher expression level of genes between mature osteoblasts and endothelial cells from the angiopoietin 2-integrin alpha 5 beta 1 pathway. Taken together, HBO therapy promotes the healing process of craniectomy by regulating the crosstalk between vascular endothelial cells and osteogenic cells. These findings provide evidence in a preclinical model that HBO therapy enhances osteointegration by regulating angiogenesis-osteogenesis coupling, providing a scientific basis for utilizing HBO therapy for accelerating postoperative recovery after craniectomy.

angiogenesis-osteogenesis coupling angiopoietin-2 craniectomy hyperbaric oxygen single-cell RNA sequencing

SCI

英语

第一 ; 通讯

SUSTech Core Research Core Research Facilities[2021A1515011447] ; Guangdong Natural Science Foundation["KQTD20200820113012029","JCYJ20190809114209434"] ; Shenzhen Science and Technology Innovation Commission[2022B1212010003]

Orthopedics

Orthopedics

WOS:001223279500001

WILEY

CLINICAL MEDICINE

Web of Science

1.Southern Univ Sci & Technol, Coll Engn, Dept Biomed Engn, Shenzhen, Peoples R China
2.Southern Univ Sci & Technol, Guangdong Prov Key Lab Adv Biomat, Shenzhen, Peoples R China
3.Southern Univ Sci & Technol, Sch Med, Shenzhen, Peoples R China
4.Shenzhen Childrens Hosp, Shenzhen, Peoples R China
5.Coll Engn Bldg,1088 Xueyuan Ave,South Room 732, Shenzhen 518055, Peoples R China
6.7019 Yitian Rd, Shenzhen 518026, Peoples R China

Pan, Yonghao,Li, Jiawei,Wu, Jianqun,et al. Hyperbaric oxygen therapy enhances osteointegration of reimplanted cranial flap by regulating osteogenesis-angiogenesis coupling[J]. JOURNAL OF ORTHOPAEDIC RESEARCH,2024.

Pan, Yonghao.,Li, Jiawei.,Wu, Jianqun.,Yang, Chengyu.,Wu, Siying.,...&Liu, Chao.(2024).Hyperbaric oxygen therapy enhances osteointegration of reimplanted cranial flap by regulating osteogenesis-angiogenesis coupling.JOURNAL OF ORTHOPAEDIC RESEARCH.

Pan, Yonghao,et al."Hyperbaric oxygen therapy enhances osteointegration of reimplanted cranial flap by regulating osteogenesis-angiogenesis coupling".JOURNAL OF ORTHOPAEDIC RESEARCH (2024).