MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR- /- mice

Shi, Jia1,8; Yang, Ming Ming6,7; Yang, Shu4; Fan, Fangyang1; Zheng, Guobin2,3; Miao, Yaodong9; Hua, Yunqing1; Zhang, Jing1; Cheng, Yanfei1; Liu, Shangjing1; Guo, Yuying1; Guo, Liping8; Yang, Xiaoxiao5; Fan, Guanwei1; Ma, Chuanrui1

2024-06-01

10.1016/j.phymed.2024.155489

PHYTOMEDICINE   影响因子和分区

0944-7113

1618-095X

Background and Purpose: Atherosclerosis is the primary pathological basis of cardiovascular disease. Ferroptosis is a regulated form of cell death, a process of lipid peroxidation driven by iron, which can initiate and promote atherosclerosis. STAT6 is a signal transducer that shows a potential role in regulating ferroptosis, but, the exact role in ferroptosis during atherogenesis remains unclear. The Traditional Chinese Medicine Maijitong granule (MJT) is used for treating cardiovascular disease and shows a potential inhibitory effect on ferroptosis. However, the antiatherogenic effect and the underlying mechanism remain unclear. In this study, we determined the role of STAT6 in ferroptosis during atherogenesis, investigated the antiatherogenic effect of MJT, and determined whether its antiatherogenic effect was dependent on the inhibition of ferroptosis. Methods: 8-week-old male LDLR -/- mice were fed a high-fat diet (HFD) at 1st and 10th week, respectively, to assess the preventive and therapeutic effects of MJT on atherosclerosis and ferroptosis. Simultaneously, the antiferroptotic effects and mechanism of MJT were determined by evaluating the expression of genes responsible for lipid peroxidation and iron metabolism. Subsequently, we reanalyzed microarray data in the GSE28117 obtained from cells after STAT6 knockdown or overexpression and analyzed the correlation between STAT6 and ferroptosis. Finally, the STAT6 -/- mice were fed HFD and injected with AAV-PCSK9 to validate the role of STAT6 in ferroptosis during atherogenesis and revealed the antiatherogenic and anti-ferroptotic effect of MJT. Results: MJT attenuated atherosclerosis by reducing plaque lesion area and enhancing plaque stability in both preventive and therapeutic groups. MJT reduced inflammation via suppressing inflammatory cytokines and inhibited foam cell formation by lowering the LDL level and promoting ABCA1/G1-mediated lipid efflux. MJT ameliorated the ferroptosis by reducing lipid peroxidation and iron dysregulation during atherogenesis. Mechanistically, STAT6 negatively regulated ferroptosis by transcriptionally suppressing SOCS1/p53 and DMT1 pathways. MJT suppressed the DMT1 and SOCS1/p53 via stimulating STAT6 phosphorylation. In addition, STAT6 knockout exacerbated atherosclerosis and ferroptosis, which abolished the antiatherogenic and antiferroptotic effects of MJT. Conclusion: STAT6 acts as a negative regulator of ferroptosis and atherosclerosis via transcriptionally suppressing DMT1 and SOCS1 expression and MJT attenuates atherosclerosis and ferroptosis by activating the STAT6mediated inhibition of DMT1 and SOCS1/p53 pathways, which indicated that STAT6 acts a novel promising therapeutic target to ameliorate atherosclerosis by inhibiting ferroptosis and MJT can serve as a new therapy for atherosclerosis treatment.

MJT Atherosclerosis Ferroptosis STAT6 DMT1 SOCS1/p53

SCI

英语

其他

Program of National Administration of Traditional Chinese Medicine["ZYYCXTD-D-202207","ZYYCXTD-C-202203"] ; Tianjin College Students Innovation and Entrepreneurship Training Program[202210063008]

Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine

Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy

WOS:001223259700001

ELSEVIER GMBH

PHARMACOLOGY & TOXICOLOGY

Web of Science

1.Tianjin Univ Tradit Chinese Med, Teaching Hosp 1, Natl Clin Res Ctr Chinese Med Acupuncture & Moxibu, 88Chang Ling Rd, Tianjin, Peoples R China
2.Tianjin Med Univ, Chu Hsien I Mem Hosp, NHC Key Lab Hormones & Dev, Tianjin Key Lab Metab Dis, Tianjin 300134, Peoples R China
3.Tianjin Med Univ, Tianjin Inst Endocrinol, Tianjin 300134, Peoples R China
4.Southern Univ Sci & Technol, Affiliated Hosp 1, Jinan Univ,Shenzhen Clin Res Ctr Geriatr, Shenzhen Peoples Hosp,Clin Med Coll 2,Guangdong Pr, Shenzhen 518020, Guangdong, Peoples R China
5.Hefei Univ Technol, Sch Food & Biol Engn, Dept Educ, Key Lab Major Metab Dis & Nutr Regulat Anhui, Hefei, Peoples R China
6.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Ophthalmol, Shenzhen 518020, Peoples R China
7.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Peoples R China
8.Tianjin Acad Tradit Chinese Med, Affiliated Hosp, Tianjin, Peoples R China
9.Tianjin Univ Tradit Chinese Med, Affiliated Hosp 2, Tianjin, Peoples R China

Shi, Jia,Yang, Ming Ming,Yang, Shu,et al. MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR- /- mice[J]. PHYTOMEDICINE,2024,128.

Shi, Jia.,Yang, Ming Ming.,Yang, Shu.,Fan, Fangyang.,Zheng, Guobin.,...&Ma, Chuanrui.(2024).MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR- /- mice.PHYTOMEDICINE,128.

Shi, Jia,et al."MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR- /- mice".PHYTOMEDICINE 128(2024).