| 题名 | Sensing antibody functions with a novel CCR8-responsive engineered cell |
| 作者 | |
| 通讯作者 | Li, Lidan; Yu, Changyuan |
| 发表日期 | 2024-04-29
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| DOI | |
| 发表期刊 | |
| EISSN | 1734-154X
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| 卷号 | 71 |
| 摘要 | Human chemokine receptor 8 (CCR8) is a promising drug target for immunotherapy of cancer and autoimmune diseases. Monoclonal antibody-based CCR8 targeted treatment shows significant inhibition in tumor growth. The inhibition of CCR8 results in the improvement of antitumor immunity and patient survival rates by regulating tumor-resident regulatory T cells. Recently monoclonal antibody drug development targeting CCR8 has become a research hotspot, which also promotes the advancement of antibody evaluation methods. Therefore, we constructed a novel engineered customized cell line HEK293-cAMP-biosensor-CCR8 combined with CCR8 and a cAMP-biosensor reporter. It can be used for the detection of anti-CCR8 antibody functions like specificity and biological activity, in addition to the detection of antibody-dependent cell-mediated cytotoxicity and antibody-dependent-cellular-phagocytosis. We obtained a new CCR8 mAb 22H9 and successfully verified its biological activities with HEK293-cAMP-biosensor-CCR8. Our reporter cell line has high sensitivity and specificity, and also offers a rapid kinetic detection platform for evaluating anti-CCR8 antibody functions. |
| 关键词 | |
| 相关链接 | [来源记录] |
| 收录类别 | |
| 语种 | 英语
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| 学校署名 | 通讯
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| 资助项目 | National Natural Science Foundation of China[82174531]
; Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties[SZGSP010]
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| WOS研究方向 | Biochemistry & Molecular Biology
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| WOS类目 | Biochemistry & Molecular Biology
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| WOS记录号 | WOS:001221473900001
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| 出版者 | |
| ESI学科分类 | BIOLOGY & BIOCHEMISTRY
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| 来源库 | Web of Science
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| 引用统计 | |
| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/788476 |
| 专题 | 南方科技大学第二附属医院 南方科技大学第一附属医院 |
| 作者单位 | 1.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing, Peoples R China 2.Jiangsu Normal Univ, Jiangsu Key Lab Phylogen & Comparat Genom, Xuzhou, Peoples R China 3.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, Affiliated Hosp 2, Shenzhen 518112, Guangdong, Peoples R China |
| 通讯作者单位 | 南方科技大学第二附属医院; 南方科技大学第一附属医院 |
| 推荐引用方式 GB/T 7714 |
Hao, Jianyu,Lv, Yitong,Xiao, Xufeng,et al. Sensing antibody functions with a novel CCR8-responsive engineered cell[J]. ACTA BIOCHIMICA POLONICA,2024,71.
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| APA |
Hao, Jianyu,Lv, Yitong,Xiao, Xufeng,Li, Lidan,&Yu, Changyuan.(2024).Sensing antibody functions with a novel CCR8-responsive engineered cell.ACTA BIOCHIMICA POLONICA,71.
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| MLA |
Hao, Jianyu,et al."Sensing antibody functions with a novel CCR8-responsive engineered cell".ACTA BIOCHIMICA POLONICA 71(2024).
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| 条目包含的文件 | 条目无相关文件。 | |||||
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