Receptor for Activated C Kinase 1 counteracts ABSCISIC ACID INSENSITIVE5-mediated inhibition of seed germination and post-germinative growth in Arabidopsis

Li, Zhiyong1,2,3; Zhang, Dayan1; Liang, Xiaoju1,4; Liang, Jiansheng1,2

2024-04-01

10.1093/jxb/erae153

JOURNAL OF EXPERIMENTAL BOTANY   影响因子和分区

0022-0957

1460-2431

["ABSCISIC ACID INSENSITIVE5 (ABI5), a key regulator of the abscisic acid (ABA) signalling pathway, plays a fundamental role in seed germination and post-germinative development. However, the molecular mechanism underlying the repression function of ABI5 remains to be elucidated. In this study, we demonstrate that the conserved eukaryotic WD40 repeat protein Receptor for Activated C Kinase 1 (RACK1) is a novel negative regulator of ABI5 in Arabidopsis. The RACK1 loss-of-function mutant is hypersensitive to ABA, while this phenotype is rescued by a mutation in ABI5. Moreover, overexpression of RACK1 suppresses ABI5 transcriptional activation activity for ABI5-targeted genes. RACK1 may also physically interact with ABI5 and facilitate its degradation. Furthermore, we found that RACK1 and the two substrate receptors CUL4-based E3 ligases (DWA1 and DWA2) function together to mediate the turnover of ABI5, thereby efficiently reducing ABA signalling in seed germination and post-germinative growth. In addition, molecular analyses demonstrated that ABI5 may bind to the promoter of RACK1 to repress its expression. Collectively, our findings suggest that RACK1 and ABI5 might form a feedback loop to regulate the homeostasis of ABA signalling in acute seed germination and early plant development.","The conserved scaffold protein Receptor for Activated C Kinase 1 negatively regulates ABA signalling through interacting with and inhibiting ABI5 in seed germination and early development in Arabidopsis."]

Abscisic acid ABI5 post-germinative growth protein degradation RACK1 seed germination

SCI

英语

第一 ; 通讯

National Natural Science Foundation of China[32070292] ; Shenzhen Science and Technology Program["KQTD20190929173906742","ZDSYS20230626091659010"] ; Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes[2019KSYS006]

Plant Sciences

Plant Sciences

WOS:001219733500001

OXFORD UNIV PRESS

PLANT & ANIMAL SCIENCE

Web of Science

1.Southern Univ Sci & Technol, Dept Biol, Shenzhen 518055, Peoples R China
2.Southern Univ Sci & Technol, Inst Plant & Food Sci, Dept Biol, Shenzhen Key Lab Plant Genet Engn & Mol Design, Shenzhen 518055, Peoples R China
3.Southern Univ Sci & Technol, Acad Adv Interdisciplinary Studies, Shenzhen 518055, Peoples R China
4.Fujian Agr & Forest Univ, Coll Life Sci, Fuzhou 350002, Peoples R China

Li, Zhiyong,Zhang, Dayan,Liang, Xiaoju,et al. Receptor for Activated C Kinase 1 counteracts ABSCISIC ACID INSENSITIVE5-mediated inhibition of seed germination and post-germinative growth in Arabidopsis[J]. JOURNAL OF EXPERIMENTAL BOTANY,2024.

Li, Zhiyong,Zhang, Dayan,Liang, Xiaoju,&Liang, Jiansheng.(2024).Receptor for Activated C Kinase 1 counteracts ABSCISIC ACID INSENSITIVE5-mediated inhibition of seed germination and post-germinative growth in Arabidopsis.JOURNAL OF EXPERIMENTAL BOTANY.

Li, Zhiyong,et al."Receptor for Activated C Kinase 1 counteracts ABSCISIC ACID INSENSITIVE5-mediated inhibition of seed germination and post-germinative growth in Arabidopsis".JOURNAL OF EXPERIMENTAL BOTANY (2024).