Transcriptomic analysis of differentially alternative splicing patterns in mice with inflammatory and neuropathic pain

Zhai, Mingzhu1,2; Huang, Jiabin3,4,5; Yang, Shaomin3,4,5; Li, Na1; Zeng, Jun6; Zheng, Yi6; Sun, Wuping3,4,5; Wu, Benqing2

2024-04-01

10.1177/17448069241249455

MOLECULAR PAIN   影响因子和分区

1744-8069

Although the molecular mechanisms of chronic pain have been extensively studied, a global picture of alternatively spliced genes and events in the peripheral and central nervous systems of chronic pain is poorly understood. The current study analyzed the changing pattern of alternative splicing (AS) in mouse brain, dorsal root ganglion, and spinal cord tissue under inflammatory and neuropathic pain. In total, we identified 6495 differentially alternatively spliced (DAS) genes. The molecular functions of shared DAS genes between these two models are mainly enriched in calcium signaling pathways, synapse organization, axon regeneration, and neurodegeneration disease. Additionally, we identified 509 DAS in differentially expressed genes (DEGs) shared by these two models, accounting for a small proportion of total DEGs. Our findings supported the hypothesis that the AS has an independent regulation pattern different from transcriptional regulation. Taken together, these findings indicate that AS is one of the important molecular mechanisms of chronic pain in mammals. This study presents a global description of AS profile changes in the full path of neuropathic and inflammatory pain models, providing new insights into the underlying mechanisms of chronic pain and guiding genomic clinical diagnosis methods and rational medication.

Alternative splicing transcriptome neuropathic pain inflammatory pain brain spinal cord dorsal root ganglion

SCI

英语

第一

Neurosciences & Neurology

Neurosciences

WOS:001217429800001

SAGE PUBLICATIONS INC

Web of Science

1.Southern Univ Sci & Technol, Yantian Hosp, Shenzhen, Peoples R China
2.Shenzhen Guangming Dist Peoples Hosp, Benqing Lab, 4253 Songbai Road, Shenzhen 518106, Peoples R China
3.Shenzhen Nanshan Peoples Hosp, Dept Pain Med, 89 Taoyuan Rd, Shenzhen 518060, Peoples R China
4.Shenzhen Nanshan Peoples Hosp, Shenzhen Municipal Key Lab Pain Med, 89 Taoyuan Rd, Shenzhen 518060, Peoples R China
5.Shenzhen Univ, Affiliated Hosp 6, Hlth Sci Ctr, 89 Taoyuan Rd, Shenzhen 518060, Peoples R China
6.Shenzhen Guangming Dist Peoples Hosp, Ctr Med Expt CME, Shenzhen, Peoples R China

Zhai, Mingzhu,Huang, Jiabin,Yang, Shaomin,et al. Transcriptomic analysis of differentially alternative splicing patterns in mice with inflammatory and neuropathic pain[J]. MOLECULAR PAIN,2024,20.

Zhai, Mingzhu.,Huang, Jiabin.,Yang, Shaomin.,Li, Na.,Zeng, Jun.,...&Wu, Benqing.(2024).Transcriptomic analysis of differentially alternative splicing patterns in mice with inflammatory and neuropathic pain.MOLECULAR PAIN,20.

Zhai, Mingzhu,et al."Transcriptomic analysis of differentially alternative splicing patterns in mice with inflammatory and neuropathic pain".MOLECULAR PAIN 20(2024).