Implication of Stm1 in the protection of eIF5A, eEF2 and tRNA through dormant ribosomes

Du, Mengtan1,2; Li, Xin1,2; Dong, Wanlin1,2; Zeng, Fuxing1,2

2024-04-18

10.3389/fmolb.2024.1395220

FRONTIERS IN MOLECULAR BIOSCIENCES   影响因子和分区

2296-889X

Background: Dormant ribosomes are typically associated with preservation factors to protect themselves from degradation under stress conditions. Stm1/SERBP1 is one such protein that anchors the 40S and 60S subunits together. Several proteins and tRNAs bind to this complex as well, yet the molecular mechanisms remain unclear.Methods: Here, we reported the cryo-EM structures of five newly identified Stm1/SERBP1-bound ribosomes.Results: These structures highlighted that eIF5A, eEF2, and tRNA might bind to dormant ribosomes under stress to avoid their own degradation, thus facilitating protein synthesis upon the restoration of growth conditions. In addition, Ribo-seq data analysis reflected the upregulation of nutrient, metabolism, and external-stimulus-related pathways in the triangle stm1 strain, suggesting possible regulatory roles of Stm1.Discussion: The knowledge generated from the present work will facilitate in better understanding the molecular mechanism of dormant ribosomes.

stm1 SERBP1 dormant ribosome cryo-EM eEF2

SCI

英语

第一 ; 通讯

National Natural Science Foundation of China["92169111","32171200"] ; Shenzhen Science and Technology Program[JCYJ20220530115210023] ; Guangdong Basic and Applied Basic Research Foundation[2021A1515010805] ; Guangdong Program[2021QN02Y353]

Biochemistry & Molecular Biology

Biochemistry & Molecular Biology

WOS:001211037300001

FRONTIERS MEDIA SA

Web of Science

1.Southern Univ Sci & Technol, Sch Life Sci, Dept Syst Biol, Shenzhen, Peoples R China
2.Southern Univ Sci & Technol, Inst Biol Electron Microscopy, Shenzhen, Peoples R China

Du, Mengtan,Li, Xin,Dong, Wanlin,et al. Implication of Stm1 in the protection of eIF5A, eEF2 and tRNA through dormant ribosomes[J]. FRONTIERS IN MOLECULAR BIOSCIENCES,2024,11.

Du, Mengtan,Li, Xin,Dong, Wanlin,&Zeng, Fuxing.(2024).Implication of Stm1 in the protection of eIF5A, eEF2 and tRNA through dormant ribosomes.FRONTIERS IN MOLECULAR BIOSCIENCES,11.

Du, Mengtan,et al."Implication of Stm1 in the protection of eIF5A, eEF2 and tRNA through dormant ribosomes".FRONTIERS IN MOLECULAR BIOSCIENCES 11(2024).