Ultrasensitive DNA Origami Plasmon Sensor for Accurate Detection in Circulating Tumor DNAs

Chen, Zhi1,2; Meng, Changle1; Wang, Xueliang1; Chen, Jiajie1; Deng, Jiefeng5; Fan, Taojian1; Wang, Lude6; Lin, Huiling8; Huang, Hao1,7; Li, Shuang5; Sun, Shuo1; Qu, Junle1; Fan, Dianyuan1,4; Zhang, Xueji1,3; Liu, Yingxia9; Shao, Yonghong1; Zhang, Han1

2024-05-01

10.1002/lpor.202400035

LASER & PHOTONICS REVIEWS   影响因子和分区

1863-8880

1863-8899

["Early lung cancer screening by computed tomography is hampered by pulmonary nodules caused by massive COVID-19 infections, necessitating an ultrasensitive approach for the early diagnosis of lung cancers at the single-base level from circular tumor DNAs (ctDNAs). This study introduces an approach that merges DNA origami and DNA scissors technologies in a framework of surface plasmon resonance (SPR) biosensors. By combining the precision of DNA origami probes with the inherent single-base resolution of DNA scissors, this method systematically addresses the limitations of conventional SPR techniques, resulting in enhanced detection accuracy. The synergistic interplay between DNA scissors and DNA origami enables the SPR biosensors to achieve unprecedented levels of sensitivity, precision, and practical utility. This efficacy allows the precise identification of mutations, demonstrated here by detection of the T790M mutation in the EGFR gene and the G12C mutation in the KRAS gene of non-small cell lung cancer patients. With this technique, single-base resolution as well as near zeptomolar-level sensitivity is achieved. As a result, this discovery holds significant potential to advance the field of precision diagnostics.","By combining DNA origami and DNA scissors with the capabilities of surface plasmon resonance (SPR), the limitations inherent in conventional SPR techniques are transcended, resulting in an unprecedented level of accuracy in gene detection. The coordination of DNA origami probes and DNA scissors technology induces detectable shifts in refractive index, thereby amplifying SPR signals and elevating sensitivity and precision to previously unattained levels. image"]

DNA origami probes non-small cell lung cancer nucleic acid detection single-base resolutions surface plasmon resonance

SCI ; EI

英语

其他

National Natural Science Foundation of China["U21A20511","62275164","82001967"] ; Key Projects (B)of the Stability Support Program for Shenzhen Higher Education Institutions[20220810151419001] ; Science and Technology Innovation Leading Talents Program of Guangdong Province[2019TX05C343] ; Department of Education of Guangdong Province[2022ZDZX1023] ; Basic and Applied Basic Research Foundation of Guangdong Province-Regional Joint Fund-Key Projects["2022B1515120012","2019B1515120043","2022-JCJQ-JJ-0886"] ; Shenzhen Science and Technology Program["KCXFZ20230731093259009","JCYJ20230808104901003","JCYJ20200109105608771","KCXFZ20211020163814021"] ; State Key Laboratory of Luminescence and Applications["SKLA-2020-03","2021008"] ; Guangdong Natural Science Foundation[2021A1515011916] ; Plan on enhancing scientific research in Guangzhou Medical University[GZMU-SH-001] ; Guangdong Medical Science and Technology Research Fund[A2023421] ; null[2019YFB2203503] ; null[YS2022YFB3200011]

Optics ; Physics

Optics ; Physics, Applied ; Physics, Condensed Matter

WOS:001214692000001

WILEY-V C H VERLAG GMBH

Web of Science

1.Shenzhen Univ, State Key Lab Radio Frequency Heterogeneous Integr, Key Lab Optoelect Devices & Syst, Minist Educ & Guangdong Prov,Coll Phys & Optoelect, Shenzhen 518060, Peoples R China
2.Shenzhen Int Inst Biomed Res, Shenzhen 518110, Peoples R China
3.Shenzhen Univ, Sch Biomed Engn, Med Sch, Shenzhen 518060, Peoples R China
4.Shenzhen Univ, Int Collaborat Lab 2D Mat Optoelect Sci & Technol, Minist Educ, Shenzhen 518060, Peoples R China
5.Dali Univ, Coll Pharm, Dali 671000, Peoples R China
6.Nanjing Univ Chinese Med, Sch Artificial Intelligence & Informat Technol, 138 Xianling Rd, Nanjing 210023, Jiangsu, Peoples R China
7.Guangzhou Med Univ, Affiliated Hosp 6, Dept Oncol, Qingyuan 511518, Guangdong, Peoples R China
8.Univ South China, Hengyang Med Coll, Hengyang 421001, Hunan, Peoples R China
9.Southern Univ Sci & Technol, Peoples Hosp Shenzhen 3, Hosp 2, Natl Clin Res Ctr Infect Dis, Shenzhen 518112, Peoples R China

Chen, Zhi,Meng, Changle,Wang, Xueliang,et al. Ultrasensitive DNA Origami Plasmon Sensor for Accurate Detection in Circulating Tumor DNAs[J]. LASER & PHOTONICS REVIEWS,2024.

Chen, Zhi.,Meng, Changle.,Wang, Xueliang.,Chen, Jiajie.,Deng, Jiefeng.,...&Zhang, Han.(2024).Ultrasensitive DNA Origami Plasmon Sensor for Accurate Detection in Circulating Tumor DNAs.LASER & PHOTONICS REVIEWS.

Chen, Zhi,et al."Ultrasensitive DNA Origami Plasmon Sensor for Accurate Detection in Circulating Tumor DNAs".LASER & PHOTONICS REVIEWS (2024).