Initial cyclic-di-GMP upregulation triggers sporadic cellular expansion leading to improved cellular survival

Yang, Yongshuai1; Guo, Siyu1; Hong, Can-Jian1; Liang, Zhao-Xun3; Ho, Chun Loong1,2,4

2024-02-01

10.1002/biot.202300542

BIOTECHNOLOGY JOURNAL   影响因子和分区

1860-6768

1860-7314

["Bacterial second messenger c-di-GMP upregulation is associated with the transition from planktonic to sessile microbial lifestyle, inhibiting cellular motility, and virulence. However, in-depth elucidation of the cellular processes resulting from c-di-GMP upregulation has not been fully explored. Here, we report the role of upregulated cellular c-di-GMP in promoting planktonic cell growth of Escherichia coli K12 and Pseudomonas aeruginosa PAO1. We found a rapid expansion of cellular growth during initial cellular c-di-GMP upregulation, resulting in a larger planktonic bacterial population. The initial increase in c-di-GMP levels promotes bacterial swarming motility during the growth phase, which is subsequently inhibited by the continuous increase of c-di-GMP, and ultimately facilitates the formation of biofilms. We demonstrated that c-di-GMP upregulation triggers key bacterial genes linked to bacterial growth, swarming motility, and biofilm formation. These genes are mainly controlled by the master regulatory genes csgD and csrA. This study provides us a glimpse of the bacterial behavior of evading potential threats through adapting lifestyle changes via c-di-GMP regulation.","This figure shows the effect of elevated c-di-GMP on bacterial planktonic growth, swarming motility, and biofilm formation progress over time. The initial upregulation of c-di-GMP stimulates the growth of planktonic bacteria, enhances swarming motility, and promotes the formation of biofilms. image"]

bacterial growth biofilm c-di-GMP motility sporadic

SCI ; EI

英语

第一 ; 通讯

National Natural Science Foundation of China's Research Fund[22050410270] ; National Natural Science Foundation of China's Research Fund for International Young Scientists[KQTD20170810111314625]

Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology

Biochemical Research Methods ; Biotechnology & Applied Microbiology

WOS:001172776200001

WILEY-V C H VERLAG GMBH

Web of Science

1.Southern Univ Sci & Technol SUSTech, Dept Biomed Engn, Shenzhen, Peoples R China
2.Chinese Acad Sci, Shenzhen Inst Adv Technol SIAT, Shenzhen Inst Synthet Biol, Shenzhen, Peoples R China
3.Nanyang Technol Univ, Sch Biol Sci, Div Struct Biol & Biochem, Singapore, Singapore
4.Southern Univ Sci & Technol SUSTech, Dept Biomed Engn, Shenzhen 518055, Peoples R China

Yang, Yongshuai,Guo, Siyu,Hong, Can-Jian,et al. Initial cyclic-di-GMP upregulation triggers sporadic cellular expansion leading to improved cellular survival[J]. BIOTECHNOLOGY JOURNAL,2024,19(2).

Yang, Yongshuai,Guo, Siyu,Hong, Can-Jian,Liang, Zhao-Xun,&Ho, Chun Loong.(2024).Initial cyclic-di-GMP upregulation triggers sporadic cellular expansion leading to improved cellular survival.BIOTECHNOLOGY JOURNAL,19(2).

Yang, Yongshuai,et al."Initial cyclic-di-GMP upregulation triggers sporadic cellular expansion leading to improved cellular survival".BIOTECHNOLOGY JOURNAL 19.2(2024).