Molecular basis for curvature formation in SepF polymerization

Liu, Wenjing1,2; Zhang, Chang1; Zhang, Huawei3,4; Ma, Shaojie1; Deng, Jing1; Wang, Daping4,5; Chang, Ziwei1; Yang, Jun1,6

2024-02-27

10.1073/pnas.2316922121

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   影响因子和分区

0027-8424

1091-6490

The self- assembly of proteins into curved structures plays an important role in many cellular processes. One good example of this phenomenon is observed in the septum- forming protein (SepF), which forms polymerized structures with uniform curvatures. SepF is essential for regulating the thickness of the septum during bacteria cell division. In Bacillus subtilis, SepF polymerization involves two distinct interfaces, the (3-(3 and alpha-alpha interfaces, which define the assembly unit and contact interfaces, respectively. However, the mechanism of curvature formation in this step is not yet fully understood. In this study, we employed solid - state NMR (SSNMR) to compare the structures of cyclic wild - type SepF assemblies with linear assemblies resulting from a mutation of G137 on the (3-(3 interface. Our results demonstrate that while the sequence differences arise from the internal assembly unit, the dramatic changes in the shape of the assemblies depend on the alpha-alpha interface between the units. We further provide atomic - level insights into how the angular variation of the alpha 2 helix on the alpha-alpha interface affects the curvature of the assemblies, using a combination of SSNMR, cryo- electron microscopy, and simulation methods. Our findings shed light on the shape control of protein assemblies and emphasize the importance of interhelical contacts in retaining curvature.

self- assembly solid- state NMR curvature cell division interface

SCI

英语

其他

National Natural Science Foundation of China["21927801","21991801","21921004","31900046","81972085","82172465"] ; Chinese Academy of Sciences[YJKYYQ20190032] ; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:001206871000003

NATL ACAD SCIENCES

BIOLOGY & BIOCHEMISTRY ; CLINICAL MEDICINE ; MULTIDISCIPLINARY ; PLANT & ANIMAL SCIENCE ; ENVIRONMENT/ECOLOGY ; SOCIAL SCIENCES, GENERAL ; MICROBIOLOGY ; ECONOMICS BUSINESS ; IMMUNOLOGY ; MATERIALS SCIENCE ; MATHEMATICS ; SPACE SCIENCE ; MOLECULAR BIOLOGY & GENETICS ; PHARMACOLOGY & TOXICOLOGY ; CHEMISTRY ; PSYCHIATRY/PSYCHOLOGY ; NEUROSCIENCE & BEHAVIOR ; PHYSICS ; GEOSCIENCES ; AGRICULTURAL SCIENCES ; ENGINEERING

Web of Science

1.Chinese Acad Sci, Wuhan Inst Phys & Math, Innovat Acad Precis Measurement Sci & Technol, Natl Ctr Magnet Resonance Wuhan,Key Lab Magnet Res, Wuhan 430071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China
4.Southern Univ Sci & Technol, Shenzhen 518055, Peoples R China
5.Shenzhen Univ, Shenzhen Peoples Hosp 2, Affiliated Hosp 1, Dept Orthoped,Guangdong Prov Res Ctr Artificial In, Shenzhen 518000, Peoples R China
6.Wuhan Univ Sci & Technol, Interdisciplinary Inst NMR & Mol Sci, Sch Chem & Chem Engn, State Key Lab Refractories & Met, Wuhan 430081, Peoples R China

Liu, Wenjing,Zhang, Chang,Zhang, Huawei,et al. Molecular basis for curvature formation in SepF polymerization[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2024,121(9).

Liu, Wenjing.,Zhang, Chang.,Zhang, Huawei.,Ma, Shaojie.,Deng, Jing.,...&Yang, Jun.(2024).Molecular basis for curvature formation in SepF polymerization.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,121(9).

Liu, Wenjing,et al."Molecular basis for curvature formation in SepF polymerization".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 121.9(2024).