Global dynamics and cytokinin participation of salt gland development trajectory in recretohalophyte Limonium bicolor

Zhao, Boqing1; Gao, Yaru1; Ma, Qiuyu1; Wang, Xi1; Zhu, Jian-Kang2,3; Li, Weiqiang4; Wang, Baoshan1,5; Yuan, Fang1,5

2024-04-23

10.1093/plphys/kiae199

PLANT PHYSIOLOGY   影响因子和分区

0032-0889

1532-2548

["Salt gland is an epidermal Na+ secretory structure that enhances salt resistance in the recretohalophyte sea lavender (Limonium bicolor). To elucidate the salt gland development trajectory and related molecular mechanisms, we performed single-cell RNA sequencing of L. bicolor protoplasts from young leaves at salt gland initiation and differentiation stages. Dimensionality reduction analyses defined 19 transcriptionally distinct cell clusters, which were assigned into 4 broad populations-promeristem, epidermis, mesophyll, and vascular tissue-verified by in situ hybridization. Cytokinin was further proposed to participate in salt gland development by the expression patterns of related genes and cytological evidence. By comparison analyses of Single-cell RNA sequencing with exogenous application of 6-benzylaminopurine, we delineated 5 salt gland development-associated subclusters and defined salt gland-specific differentiation trajectories from Subclusters 8, 4, and 6 to Subcluster 3 and 1. Additionally, we validated the participation of TRIPTYCHON and the interacting protein Lb7G34824 in salt gland development, which regulated the expression of cytokinin metabolism and signaling-related genes such as GLABROUS INFLORESCENCE STEMS 2 to maintain cytokinin homeostasis during salt gland development. Our results generated a gene expression map of young leaves at single-cell resolution for the comprehensive investigation of salt gland determinants and cytokinin participation that helps elucidate cell fate determination during epidermis formation and evolution in recretohalophytes.","Single-cell RNA sequencing of Limonium bicolor protoplasts reveals global dynamics of salt gland development and that cytokinin participates in salt gland development trajectory."]

SCI

英语

其他

National Natural Science Research Foundation of China (NSFC)["32370304","32170301"] ; MOE Layout Foundation of Humanities and Social Sciences[21YJAZH108]

Plant Sciences

Plant Sciences

WOS:001206419900001

OXFORD UNIV PRESS INC

PLANT & ANIMAL SCIENCE

Web of Science

1.Shandong Normal Univ, Coll Life Sci, Shandong Prov Key Lab Plant Stress, Jinan 250014, Peoples R China
2.Southern Univ Sci & Technol, Inst Adv Biotechnol, Shenzhen 518055, Peoples R China
3.Southern Univ Sci & Technol, Sch Med, Shenzhen 518055, Peoples R China
4.Chinese Acad Sci, Jilin Daan Agroecosyst Natl Observat Res Stn, Changchun Jingyuetan Remote Sensing Expt Stn, State Key Lab Black Soils Conservat & Utilizat,Nor, Changchun 130102, Peoples R China
5.Shandong Normal Univ, Dongying Inst, Dongying Key Lab Salt Tolerance Mech & Applicat Ha, Dongying 257000, Peoples R China

Zhao, Boqing,Gao, Yaru,Ma, Qiuyu,et al. Global dynamics and cytokinin participation of salt gland development trajectory in recretohalophyte Limonium bicolor[J]. PLANT PHYSIOLOGY,2024,195(3).

Zhao, Boqing.,Gao, Yaru.,Ma, Qiuyu.,Wang, Xi.,Zhu, Jian-Kang.,...&Yuan, Fang.(2024).Global dynamics and cytokinin participation of salt gland development trajectory in recretohalophyte Limonium bicolor.PLANT PHYSIOLOGY,195(3).

Zhao, Boqing,et al."Global dynamics and cytokinin participation of salt gland development trajectory in recretohalophyte Limonium bicolor".PLANT PHYSIOLOGY 195.3(2024).