南方科技大学知识苑(SUSTech KC): Photoacoustic expansion microscopy of melanosomes

题名	Photoacoustic expansion microscopy of melanosomes
作者	Li, Tingting 1 ; Gong, Xinrui 1; Guo, Heng1 ; Xi, Lei1,2
通讯作者	Xi, Lei
发表日期	2024-02-15
DOI	10.1364/OL.509831
发表期刊	OPTICS LETTERS 影响因子和分区
ISSN	0146-9592
EISSN	1539-4794
卷号	49 期号:4
摘要	Optical resolution photoacoustic microscopy (OR-PAM) is a hybrid imaging method for visualizing organelles due to the high spatial resolution and abundant optical contrast. Usually, OR-PAM employs high numerical aperture (NA) objectives and high-frequency ultrasonic detectors to resolve three-dimensional (3D) microstructures of cells. Expansion microscopy (ExM) provides a nanoscale resolution by isotropically enlarging cells instead of utilizing ultrahigh NA objectives. In this Letter, we report the development of photoacoustic expansion microscopy (PA-ExM) that combines the advantages of OR-PAM and ExM for 3D organelle imaging using near-infrared light. We evaluate the performance of PA-ExM using label -free melanoma cells, where the image quality of melanosome distributions in expanded cells using a 40x objective is comparable to that of unexpanded cells using an oil-immersed 100x objective. The results suggest that PA-ExM possesses the great potential to study organelles. (c) 2024 Optica Publishing Group
相关链接	[来源记录]
收录类别	SCI ; EI
语种	英语
学校署名	第一 ; 通讯
资助项目	National Natural Science Foundation of China["62022037","62305148","61775028","81571722","61528401"] ; Guangdong Science and Technology Department["2019ZT08Y191","2022B1212010003"] ; Shenzhen Science and Technology Program["JCYJ20230807093105010","JCYJ20200109141222892","KQTD20190929172743294"] ; Startup grant from Southern University of Science and Technology[PDJH2021C008]
WOS研究方向	Optics
WOS类目	Optics
WOS记录号	WOS:001199852800004
出版者	Optica Publishing Group
ESI学科分类	PHYSICS
来源库	Web of Science
引用统计
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/788619
专题	工学院_生物医学工程系南方科技大学
作者单位	1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China 2.Southern Univ Sci & Technol, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Guangdong, Peoples R China
第一作者单位	生物医学工程系
通讯作者单位	生物医学工程系; 南方科技大学
第一作者的第一单位	生物医学工程系
推荐引用方式 GB/T 7714	Li, Tingting,Gong, Xinrui,Guo, Heng,et al. Photoacoustic expansion microscopy of melanosomes[J]. OPTICS LETTERS,2024,49(4).
APA	Li, Tingting,Gong, Xinrui,Guo, Heng,&Xi, Lei.(2024).Photoacoustic expansion microscopy of melanosomes.OPTICS LETTERS,49(4).
MLA	Li, Tingting,et al."Photoacoustic expansion microscopy of melanosomes".OPTICS LETTERS 49.4(2024).