Surface-enhanced Raman scattering spatial fingerprinting decodes the digestion behavior of lysosomes in live single cells

Liu, Fugang1; Sun, Zhirui2; Li, Bingyi1; Liu, Jiaqing1; Chen, Zhou1; Ye, Jian1,3,4,5,6

2024-06-01

10.1002/VIW.20240004

VIEW   影响因子和分区

2688-3988

2688-268X

["Lysosome, the digestive organelle in eukaryotic cells, plays an important role in the degradation and recirculation of cellular products as well as in maintaining the stability of cellular metabolic microenvironment. Surface-enhanced Raman scattering (SERS) is a molecular fingerprint technology with high detection sensitivity and photostability, suited for revealing various intracellular molecular information by inducing endocytosis of SERS-active nanoparticles. However, it remains challenging to selectively extract the molecular information of specific organelles (e.g., lysosomes) from a high-dimensional spectral set. Herein, we proposed a novel paradigm by combining label-free SERS spectroscopy with confocal fluorescence imaging to investigate the digestion behavior of lysosomes in cells. The structural similarity algorithm was innovatively introduced and exhibited its effectiveness in screening out the wavenumbers in the SERS spectral set with high correlation with the metabolic behaviors of lysosomes. With comprehensive experiments on HeLa single cells, we captured the intracellular macromolecular digestion phenomenon and discovered the changing pattern of cellular SERS spectra after starvation-induced autophagy, and analyzed the molecular information within the lysosomes in three-dimensional space.","The authors have developed an approach by combining label-free surface-enhanced Raman spectroscopy with confocal fluorescence imaging to decode the digestion behavior of lysosomes in live single cells with the assistance of the structural similarity algorithm. image"]

lysosomal digestion nanoparticles structural similarity surface-enhanced Raman scattering

ESCI ; EI

英语

其他

Science and Technology Commission of Shanghai Municipality[21511102100] ; Shanghai Jiao Tong University[YG2024LC09] ; null[82272054]

Science & Technology - Other Topics ; Materials Science

Nanoscience & Nanotechnology ; Materials Science, Biomaterials

WOS:001205008500001

WILEY

Web of Science

1.Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200030, Peoples R China
2.Southern Univ Sci & Technol, Dept Elect & Elect Engn, Shenzhen, Peoples R China
3.Shanghai Jiao Tong Univ, Ren Ji Hosp, Sch Med, State Key Lab Syst Med Canc, Shanghai, Peoples R China
4.Shanghai Jiao Tong Univ, Inst Med Robot, Shanghai, Peoples R China
5.Shanghai Jiao Tong Univ, Ren Ji Hosp, Sch Med, Shanghai Key Lab Gynecol Oncol, Shanghai, Peoples R China
6.Shanghai Jiao Tong Univ, Natl Engn Res Ctr Adv Magnet Resonance Technol Dia, Sch Biomed Engn, Shanghai, Peoples R China

Liu, Fugang,Sun, Zhirui,Li, Bingyi,et al. Surface-enhanced Raman scattering spatial fingerprinting decodes the digestion behavior of lysosomes in live single cells[J]. VIEW,2024,5(3).

Liu, Fugang,Sun, Zhirui,Li, Bingyi,Liu, Jiaqing,Chen, Zhou,&Ye, Jian.(2024).Surface-enhanced Raman scattering spatial fingerprinting decodes the digestion behavior of lysosomes in live single cells.VIEW,5(3).

Liu, Fugang,et al."Surface-enhanced Raman scattering spatial fingerprinting decodes the digestion behavior of lysosomes in live single cells".VIEW 5.3(2024).