Knockout of miR396 genes increases seed size and yield in soybean^â

Xie, Hongtao1,2,3; Su, Fei4; Niu, Qingfeng1,2; Geng, Leping1,2; Cao, Xuesong5; Song, Minglei5; Dong, Jinsong4; Zheng, Zai6; Guo, Rui4; Zhang, Yang4; Deng, Yuanwei1,2; Ji, Zhanbo3; Pang, Kang3; Zhu, Jian-Kang5; Zhu, Jianhua1,2

2024-06-01

10.1111/jipb.13660

JOURNAL OF INTEGRATIVE PLANT BIOLOGY   影响因子和分区

1672-9072

1744-7909

Yield improvement has long been an important task for soybean breeding in the world in order to meet the increasing demand for food and animal feed. miR396 genes have been shown to negatively regulate grain size in rice, but whether miR396 family members may function in a similar manner in soybean is unknown. Here, we generated eight soybean mutants harboring different combinations of homozygous mutations in the six soybean miR396 genes through genome editing with clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated nuclease (Cas)12SF01 in the elite soybean cultivar Zhonghuang 302 (ZH302). Four triple mutants (mir396aci, mir396acd, mir396adf, and mir396cdf), two quadruple mutants (mir396abcd and mir396acfi), and two quintuple mutants (mir396abcdf and mir396bcdfi) were characterized. We found that plants of all the mir396 mutants produced larger seeds compared to ZH302 plants. Field tests showed that mir396adf and mir396cdf plants have significantly increased yield in growth zones with relatively high latitude which are suited for ZH302 and moderately increased yield in lower latitude. In contrast, mir396abcdf and mir396bcdfi plants have increased plant height and decreased yield in growth zones with relatively high latitude due to lodging issues, but they are suited for low latitude growth zones with increased yield without lodging problems. Taken together, our study demonstrated that loss-of-function of miR396 genes leads to significantly enlarged seed size and increased yield in soybean, providing valuable germplasms for breeding high-yield soybean.

Cas12SF01 CRISPR/Cas miR396 seed size soybean yield

SCI

英语

通讯

National Key Research and Development Program of China[NK2022010301] ; Key Research & Development Program of Shandong Province, China[2021LZGC012] ; Innovative research team of Anhui Education[2022AH010056] ; National Natural Science Foundation of China[32270367] ; PhD Scientific Research and Innovation Foundation of Sanya Yazhou Bay Science and Technology City[HSPHDSRF-2023-11-007] ; null[2021LZGC003]

Biochemistry & Molecular Biology ; Plant Sciences

Biochemistry & Molecular Biology ; Plant Sciences

WOS:001199796700001

WILEY

Web of Science

1.Anhui Agr Univ, Sch Life Sci, Hefei 230036, Peoples R China
2.Anhui Agr Univ, Res Inst Frontier Sci, Res Ctr Biol Breeding Technol, Hefei 230036, Peoples R China
3.Bellagen Biotechnol Co Ltd, Jinan 250000, Peoples R China
4.Chinese Acad Agr Sci, Natl Nanfan Res Inst, Inst Crop Sci, Minist Agr & Rural Affairs,Key Lab Gene Editing Te, Sanya 572025, Peoples R China
5.Southern Univ Sci & Technol, Shenzhen 518055, Peoples R China
6.Hainan Yazhou Bay Seed Lab, Sanya 572024, Peoples R China

Xie, Hongtao,Su, Fei,Niu, Qingfeng,et al. Knockout of miR396 genes increases seed size and yield in soybean^â [J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2024,66(6).

Xie, Hongtao.,Su, Fei.,Niu, Qingfeng.,Geng, Leping.,Cao, Xuesong.,...&Zhu, Jianhua.(2024).Knockout of miR396 genes increases seed size and yield in soybean^â .JOURNAL OF INTEGRATIVE PLANT BIOLOGY,66(6).

Xie, Hongtao,et al."Knockout of miR396 genes increases seed size and yield in soybean^â ".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 66.6(2024).