A high-performance cell-labeling NIR-II dye for in vivo cell tracking

Sun, Bin1,2; Ma, Rui3; Wang, Xin1; Ma, Shengjie1; Li, Wenzhong1; Liu, Tianyi4; Zhu, Wenhao4; Ji, Zhengchao1; Hettie, Kenneth S.5; Liu, Chunchen3,7; Liang, Yongye3,7; Zhu, Shoujun1,2,6

2024-04-01

10.1002/VIW.20230097

VIEW   影响因子和分区

2688-3988

2688-268X

["Fluorescent dyes that emit in the second near-infrared (NIR-II, 1000-3000 nm) region have provided significant advances toward real-time and high-resolution imaging of vessel and lymphatic system. However, in vivo NIR-II tracking of the fate of labeled cells still remains challenging. Here, we develop a shielding unit-donor-acceptor-donor-shielding unit (S-D-A-D-S) NIR-II fluorophore (FE-4ZW) with zwitterionic terminal groups for high-efficiency cell labeling without using cell-penetrating peptides, which provides for enhanced non-invasive in vivo determination of the location of cell migration. The tethering terminal sulfoammonium inner salts are featured with its high affinity for cell membranes, thereby enabling the stable labeling even for fixed cells. The fate of transplanted stem cell and the tumor cell migration along lymphatic system in brain or periphery tissues are clearly monitored by the cell-internalized FE-4ZW. We also confirmed that a clinically used surfactant, D-alpha-tocopheryl polyethylene glycol-1000 succinate, can reduce the liver and spleen uptake of FE-4ZW. The fluorophore design strategy and cell-labeling technology reported here open a new realm in the visualization of cell migration and insight into the relocation process, thereby ultimately providing an opportunity to investigate in greater detail of the underlying mechanisms of stem cell therapy and tumor metastasis.","In vivo cell tracking through a non-invasive route that affords high spatiotemporal resolution is essential for investigating the fate of transplanted cells. Here, we report NIR-II-fluorescence-emitting organic small-molecule fluorophore having an S-D-A-D-S underpinning accompanied by tethering zwitterionic terminal groups that collectively afford the high-performance cell-labeling and high-efficiency live cell tracking for using in in vivo applications. image"]

brain tumour cell migration glymphatic system high-performance cell labeling in vivo cell tracking NIR-II bioimaging organic NIR-II dye

ESCI ; EI

英语

通讯

Guangdong Provincial Natural Science Foundation[2019A1515110464] ; Shenzhen Science and Technology Innovation Commission-Free Exploration/General Project[JCYJ20190812151209348] ; Shenzhen Science and Technology Innovation Commission-Technology Breakthrough Project[JSGG20191231141403880] ; null[2022YFC2408100]

Science & Technology - Other Topics ; Materials Science

Nanoscience & Nanotechnology ; Materials Science, Biomaterials

WOS:001196051400001

WILEY

Web of Science

1.First Hosp Jilin Univ, Joint Lab Optofunct Theranost Med & Chem, Changchun, Peoples R China
2.Jilin Univ, Coll Chem, State Key Lab Supramol Struct & Mat, Changchun, Peoples R China
3.Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen, Peoples R China
4.First Hosp Jilin Univ, Dept Neurosurg, Changchun, Peoples R China
5.Stanford Univ, Sch Med, Dept Radiol & Otolaryngol Head & Neck Surg, Mol Imaging Program Stanford, Stanford, CA USA
6.First Hosp Jilin Univ, Joint Lab Optofunct Theranost Med & Chem, Changchun 130021, Peoples R China
7.Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen 518055, Peoples R China

Sun, Bin,Ma, Rui,Wang, Xin,et al. A high-performance cell-labeling NIR-II dye for in vivo cell tracking[J]. VIEW,2024,5(2).

Sun, Bin.,Ma, Rui.,Wang, Xin.,Ma, Shengjie.,Li, Wenzhong.,...&Zhu, Shoujun.(2024).A high-performance cell-labeling NIR-II dye for in vivo cell tracking.VIEW,5(2).

Sun, Bin,et al."A high-performance cell-labeling NIR-II dye for in vivo cell tracking".VIEW 5.2(2024).