Risk factors and genetic characteristics of the carriage of hypervirulent and carbapenem-resistant Acinetobacter baumannii among pregnant women

Zheng, Chao1,2,3,4; Li, Defeng1; Wang, Yinglan5; Wang, Lisheng1; Huang, Yuting2,6; Yao, Jun1

2024-03-20

10.3389/fmicb.2024.1351722

FRONTIERS IN MICROBIOLOGY   影响因子和分区

1664-302X

Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) and its emerging evolutionary branch toward hypervirulence have been neglected in pregnancy. Methods: From September 2020 to August 2021, an active surveillance culture program encompassed 138 randomly selected pregnant women, with five subjected to sample collection at two different time points. The clinical characterization was explored through statistical analysis. Whole-genome sequencing, a Galleria mellonella infection model, and a global database were used to investigate the genetic characterization, pathogenicity, evolutionary history, and phylogenetic relationships of the isolates. Results: Of the 41 CRAB isolates obtained, they were divided into four Clusters(RS) and an orphan pattern. Cluster(RS) 1 (n = 31), with eight complex types in pregnancy, was also the dominant Cluster(RS) globally, followed by Cluster(RS) 13 (n = 5), identified as hypervirulent KL49 CRAB, exhibiting phylogeographical specificity to Guangdong. A maternal carriage CRAB rate of 26.09% (36/138) was revealed, with half of the isolates representing novel complex types, prominently including CT3071, as the first KL7 isolates identified in Shenzhen. Both KL49 and KL7 isolates were most commonly found in the same participant, suggesting potential intraspecific competition as a possible reason for CRAB infection without carriers during pregnancy. The independent risk factors for carriers were revealed for the first time, including advanced maternal age, gestational diabetes mellitus, and Group B Streptococcus infection. Conclusion: The significant carriage rate and enhanced virulence of CRAB during pregnancy emphasize the imperative for routine surveillance to forestall dissemination within this high-risk group, especially in Guangdong for Cluster(RS) 13 isolates.

carbapenem-resistant Acinetobacter baumannii high virulence maternal carriage genotyping risk factors

SCI

英语

第一 ; 通讯

Microbiology

Microbiology

WOS:001195890300001

FRONTIERS MEDIA SA

Web of Science

1.Southern Univ Sci & Technol, Jinan Univ, Affiliated Hosp 1, Dept Gastroenterol,Shenzhen Peoples Hosp,Clin Med, Shenzhen, Peoples R China
2.Southern Univ Sci & Technol, Jinan Univ, Clin Med Coll 2, Bacteriol & Antibacterial Resistance Surveillance, Shenzhen, Peoples R China
3.Jinan Univ, Integrated Chinese & Western Med Postdoctoral Res, Guangzhou, Peoples R China
4.Shenzhen Polytech Univ, Sch Mat & Environm Engn, Shenzhen, Peoples R China
5.Southern Univ Sci & Technol, Jinan Univ, Clin Med Coll 2, Dept Obstet & Gynecol,Shenzhen Peoples Hosp,Affili, Shenzhen, Peoples R China
6.Chinese Acad Med Sci, Canc Hosp, Shenzhen Ctr, Dept Head & Neck Surg, Shenzhen, Peoples R China

Zheng, Chao,Li, Defeng,Wang, Yinglan,et al. Risk factors and genetic characteristics of the carriage of hypervirulent and carbapenem-resistant Acinetobacter baumannii among pregnant women[J]. FRONTIERS IN MICROBIOLOGY,2024,15.

Zheng, Chao,Li, Defeng,Wang, Yinglan,Wang, Lisheng,Huang, Yuting,&Yao, Jun.(2024).Risk factors and genetic characteristics of the carriage of hypervirulent and carbapenem-resistant Acinetobacter baumannii among pregnant women.FRONTIERS IN MICROBIOLOGY,15.

Zheng, Chao,et al."Risk factors and genetic characteristics of the carriage of hypervirulent and carbapenem-resistant Acinetobacter baumannii among pregnant women".FRONTIERS IN MICROBIOLOGY 15(2024).