RACK1A promotes hypocotyl elongation by scaffolding light signaling components in Arabidopsis

Fu, Yajuan; Zhu, Wei; Zhou, Yeling; Su, Yujing; Li, Zhiyong; Zhang, Dayan; Zhang, Dong; Shen, Jinyu; Liang, Jiansheng

2024-05-01

10.1111/jipb.13651

JOURNAL OF INTEGRATIVE PLANT BIOLOGY   影响因子和分区

1672-9072

1744-7909

Plants deploy versatile scaffold proteins to intricately modulate complex cell signaling. Among these, RACK1A (Receptors for Activated C Kinase 1A) stands out as a multifaceted scaffold protein functioning as a central integrative hub for diverse signaling pathways. However, the precise mechanisms by which RACK1A orchestrates signal transduction to optimize seedling development remain largely unclear. Here, we demonstrate that RACK1A facilitates hypocotyl elongation by functioning as a flexible platform that connects multiple key components of light signaling pathways. RACK1A interacts with PHYTOCHROME INTERACTING FACTOR (PIF)3, enhances PIF3 binding to the promoter of BBX11 and down-regulates its transcription. Furthermore, RACK1A associates with ELONGATED HYPOCOTYL 5 (HY5) to repress HY5 biochemical activity toward target genes, ultimately contributing to hypocotyl elongation. In darkness, RACK1A is targeted by CONSTITUTIVELY PHOTOMORPHOGENIC (COP)1 upon phosphorylation and subjected to COP1-mediated degradation via the 26 S proteasome system. Our findings provide new insights into how plants utilize scaffold proteins to regulate hypocotyl elongation, ensuring proper skoto- and photo-morphogenic development.

Arabidopsis hypocotyl development light signaling components RACK1 scaffold protein

SCI

英语

第一 ; 通讯

Shenzhen Science and Technology Program[KQTD20190929173906742] ; Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes["2019KSYS006","K19227561","K21227504"] ; null[32070292]

Biochemistry & Molecular Biology ; Plant Sciences

Biochemistry & Molecular Biology ; Plant Sciences

WOS:001194558100001

WILEY

Web of Science

Southern Univ Sci & Technol, Inst Plant & Food Sci, Key Lab Mol Design Plant Cell Factory, Guangdong Higher Educ Inst,Sch Life Sci,Dept Biol, Shenzhen 518055, Peoples R China

Fu, Yajuan,Zhu, Wei,Zhou, Yeling,et al. RACK1A promotes hypocotyl elongation by scaffolding light signaling components in Arabidopsis[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2024,66(5).

Fu, Yajuan.,Zhu, Wei.,Zhou, Yeling.,Su, Yujing.,Li, Zhiyong.,...&Liang, Jiansheng.(2024).RACK1A promotes hypocotyl elongation by scaffolding light signaling components in Arabidopsis.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,66(5).

Fu, Yajuan,et al."RACK1A promotes hypocotyl elongation by scaffolding light signaling components in Arabidopsis".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 66.5(2024).