One-step biofabrication of liquid core-GelMa shell microbeads for in situ hollow cell ball self-assembly

Chen, Jianwei1,2; Liu, Zeyang3; Wang, Zixian2; Zhang, Xiuxiu2; Zhang, Yi2; Zhan, Zhen3; Gong, Xiaohua4,5; Xu, Tao1,2,6

2024-01-02

10.1093/rb/rbae021

REGENERATIVE BIOMATERIALS   影响因子和分区

2056-3418

2056-3426

There are many instances of hollow-structure morphogenesis in the development of tissues. Thus, the fabrication of hollow structures in a simple, high-throughput and homogeneous manner with proper natural biomaterial combination is valuable for developmental studies and tissue engineering, while it is a significant challenge in biofabrication field. We present a novel method for the fabrication of a hollow cell module using a coaxial co-flow capillary microfluidic device. Sacrificial gelatin laden with cells in the inner layer and GelMa in the outer layer are used via a coaxial co-flow capillary microfluidic device to produce homogenous micro-beads. The overall and core sizes of core-shell microbeads were well controlled. When using human vein vascular endothelial cells to demonstrate how cells line the inner surface of core-shell beads, as the core liquifies, a hollow cell ball with asymmetric features is fabricated. After release from the GelMa shell, individual cell balls are obtained and deformed cell balls can self-recover. This platform paves way for complex hollow tissue modeling in vitro, and further modulation of matrix stiffness, curvature and biochemical composition to mimic in vivo microenvironments.

core-shell beads hollow cell ball microfluidics hydrogels soft matter

SCI ; EI

英语

其他

Key-Area Research and Development Program of Guangdong Province[2023B0909020003] ; National Natural Science Foundation of China[52075285] ; Applied Basic Research Project of Sichuan Province[2021YJ0563]

Materials Science

Materials Science, Biomaterials

WOS:001189425200001

OXFORD UNIV PRESS

Web of Science

1.Tsinghua Univ, Res Inst Tsinghua Univ Shenzhen, Biointelligent Mfg & Living Matter Bioprinting Ctr, Shenzhen 518057, Peoples R China
2.Tsinghua Univ, Tsinghua Berkeley Shenzhen Inst TBSI, Precis Med & Healthcare Res Ctr, Shenzhen 518055, Peoples R China
3.Southern Univ Sci & Technol, Dept Mech & Energy Engn, Shenzhen, 94720, Peoples R China
4.Univ Calif Berkeley, Sch Optometry, Berkeley, CA 94720 USA
5.Univ Calif Berkeley, Vis Sci Program, Berkeley, CA 94720 USA
6.Tsinghua Univ, Tsinghua Shenzhen Int Grad Sch, Shenzhen 518055, Peoples R China

Chen, Jianwei,Liu, Zeyang,Wang, Zixian,et al. One-step biofabrication of liquid core-GelMa shell microbeads for in situ hollow cell ball self-assembly[J]. REGENERATIVE BIOMATERIALS,2024,11.

Chen, Jianwei.,Liu, Zeyang.,Wang, Zixian.,Zhang, Xiuxiu.,Zhang, Yi.,...&Xu, Tao.(2024).One-step biofabrication of liquid core-GelMa shell microbeads for in situ hollow cell ball self-assembly.REGENERATIVE BIOMATERIALS,11.

Chen, Jianwei,et al."One-step biofabrication of liquid core-GelMa shell microbeads for in situ hollow cell ball self-assembly".REGENERATIVE BIOMATERIALS 11(2024).