南方科技大学知识苑(SUSTech KC): Facile Blinking Dyes with Spontaneous<i> Enol-Keto</i> Tautomerism for Super-Resolution Imaging of Subcellular Targets

题名	Facile Blinking Dyes with Spontaneous Enol-Keto Tautomerism for Super-Resolution Imaging of Subcellular Targets
作者	Gao, Ji 1 ; Wu, Weijun1 ; Zhou, Lulu1 ; Gong, Shaokuan2 ; Li, Chong1 ; Chen, Xihan2 ; Li, Yiming1 ; Li, Kai1
通讯作者	Chen, Xihan; Li, Yiming; Li, Kai
发表日期	2024-03-01
DOI	10.31635/ccschem.024.202303818
发表期刊	CCS CHEMISTRY 影响因子和分区
EISSN	2096-5745
摘要	Fluorophores with controlled on-off switching properties and subcellular targeting abilities are essential for modern super -resolution microscopy techniques. However, the effective design strategies of blinking fluorophores are still limited with scarce building blocks (e.g., rhodamine and cyanine analogues), and sophisticated functionalization is always required for organelle recognition. Therefore, exploring live -cell compatible and organelle-targetable fluorophores with a new blinking mechanism is of broad interest to promote the emerging development of superresolution microscopy. Here, we report a type of live -cell permeable fluorophore with a facile synthesis strategy to facilitate the imaging of lipid droplets and lysosomes using single -molecule localization microscopy. The fluorophores only require a two-step synthetic route. They show signature enol-keto tautomerism by spontaneous proton transfer at the ground state without additional photoactivation, which ensures blinking performance by exciting the minor portion of molecules in keto form. This fluorescence switching mechanism provides a distinct character from that of previously reported live -cell permeable dyes for super -resolution imaging, while the specific organelle binding affinity can be tuned by simply changing the targeting moiety of fluorophores.
关键词	fluorophore proton transfer fluorescence imaging subcellular target spontaneous enol-keto tautomerism
相关链接	[来源记录]
收录类别	ESCI
语种	英语
学校署名	第一 ; 通讯
资助项目	Ministry of Science and Technology of China[2020YFA0908900] ; National Natural Science Foundation of China[U21A2097] ; Key Technology Research and Development Program of Shandong[2021CXGC010212] ; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003] ; Guangdong Natural Science Foundation Joint Fund[2020A1515110380] ; Shenzhen Science and Technology Program["KQTD20200820113012029","JCYJ20220818100416036"]
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:001182444400001
出版者	CHINESE CHEMICAL SOC
来源库	Web of Science
引用统计	被引频次[WOS]：1
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/788821
专题	工学院_生物医学工程系工学院_机械与能源工程系
作者单位	1.Southern Univ Sci & Technol SUSTech, Dept Biomed Engn, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Peoples R China 2.Southern Univ Sci & Technol SUSTech, SUSTech Energy Inst Carbon Neutral, Dept Mech & Energy Engn, Shenzhen 518055, Peoples R China
第一作者单位	生物医学工程系
通讯作者单位	机械与能源工程系; 生物医学工程系
第一作者的第一单位	生物医学工程系
推荐引用方式 GB/T 7714	Gao, Ji,Wu, Weijun,Zhou, Lulu,et al. Facile Blinking Dyes with Spontaneous Enol-Keto Tautomerism for Super-Resolution Imaging of Subcellular Targets[J]. CCS CHEMISTRY,2024.
APA	Gao, Ji.,Wu, Weijun.,Zhou, Lulu.,Gong, Shaokuan.,Li, Chong.,...&Li, Kai.(2024).Facile Blinking Dyes with Spontaneous Enol-Keto Tautomerism for Super-Resolution Imaging of Subcellular Targets.CCS CHEMISTRY.
MLA	Gao, Ji,et al."Facile Blinking Dyes with Spontaneous Enol-Keto Tautomerism for Super-Resolution Imaging of Subcellular Targets".CCS CHEMISTRY (2024).