南方科技大学知识苑(SUSTech KC): Identification of circRNA-miRNA-mRNA Network Regulated by Hsp90 in Human Melanoma A375 Cells

题名	Identification of circRNA-miRNA-mRNA Network Regulated by Hsp90 in Human Melanoma A375 Cells
作者	Fu, Qiang 1; Gao, Hengyuan 1; Liu, Kaisheng 1; Su, Juan 2; Zhang, Jianglin 1,3; Guo, Xiaojing 1; Yang, Fang1,3
通讯作者	Guo, Xiaojing; Yang, Fang
发表日期	2024
DOI	10.2174/1386207326666230609145247
发表期刊	COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING 影响因子和分区
ISSN	1386-2073
EISSN	1875-5402
卷号	27 期号:2
摘要	Background Melanoma is the deadliest form of skin cancer. Heat shock protein \n90 (Hsp90) is highly expressed in human melanoma. Hsp90 inhibitors can suppress the growth of human melanoma A375 cells; however, the underlying mechanism remains unclear.Methods A375 cells were treated with SNX-2112, an Hsp90 inhibitor, for 48 h, and whole-transcriptome sequencing was performed.Results A total of 2,528 differentially expressed genes were identified, including 895 upregulated and 1,633 downregulated genes. Pathway enrichment analyses of differentially expressed mRNAs identified the extracellular matrix (ECM)-receptor interaction pathway as the most significantly enriched pathway. The ECM receptor family mainly comprises integrins (ITGs) and collagens (COLs), wherein ITGs function as the major cell receptors for COLs. 19 upregulated miRNAs were found to interact with 6 downregulated ITG genes and 8 upregulated miRNAs were found to interact with 3 downregulated COL genes. 9 differentially expressed circRNAs in SNX-2112-treated A375 cells were identified as targets of the ITG- and COL-related miRNAs. Based on the differentially expressed circRNAs, miRNAs, and mRNAs, ITGs- and COL-based circRNA-miRNA-mRNA regulatory networks were mapped, revealing a novel regulatory mechanism of Hsp90-regulated melanoma.Conclusion Targeting the ITG-COL network is a promising approach to the treatment of melanoma.
关键词	Melanoma Hsp90 SNX-2112 integrins collagens regulatory network
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	Science and Technology Foundation of Shenzhen[JCYJ20200109144410181] ; National Natural Science Foundation of China[82203941] ; Medical Scientific Research Foundation of Guangdong Province of China[A2023297]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
WOS类目	Biochemical Research Methods ; Chemistry, Applied ; Pharmacology & Pharmacy
WOS记录号	WOS:001187195100003
出版者	BENTHAM SCIENCE PUBL LTD
ESI学科分类	CHEMISTRY
来源库	Web of Science
引用统计
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/788851
专题	南方科技大学第一附属医院
作者单位	1.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Guangdong Prov Clin Res Ctr Geriatr, Jinan Univ,Affiliated Hosp 1,Shenzhen Clin Res Ctr, Shenzhen 518020, Guangdong, Peoples R China 2.Cent South Univ, Xiangya Hosp, Dept Dermatol, Changsha, Peoples R China 3.Natl Clin Res, Ctr Skin Dis, Candidate Branch, Shenzhen 518020, Guangdong, Peoples R China
第一作者单位	南方科技大学第一附属医院
通讯作者单位	南方科技大学第一附属医院
第一作者的第一单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Fu, Qiang,Gao, Hengyuan,Liu, Kaisheng,et al. Identification of circRNA-miRNA-mRNA Network Regulated by Hsp90 in Human Melanoma A375 Cells[J]. COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING,2024,27(2).
APA	Fu, Qiang.,Gao, Hengyuan.,Liu, Kaisheng.,Su, Juan.,Zhang, Jianglin.,...&Yang, Fang.(2024).Identification of circRNA-miRNA-mRNA Network Regulated by Hsp90 in Human Melanoma A375 Cells.COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING,27(2).
MLA	Fu, Qiang,et al."Identification of circRNA-miRNA-mRNA Network Regulated by Hsp90 in Human Melanoma A375 Cells".COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING 27.2(2024).