Wogonoside Ameliorates Airway Inflammation and Mucus Hypersecretion via NF-κB/STAT6 Signaling in Ovalbumin-Induced Murine Acute Asthma

Yu, Xiu; Cai, Bicheng; Yu, Li; Li, Nan; Wu, Chujie; Hu, Zhiquan; Tang, Dong; Chen, Rongchang; Qiu, Chen

2024-03-20

10.1021/acs.jafc.3c04082

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   影响因子和分区

0021-8561

1520-5118

Asthma is recognized as a chronic respiratory illness characterized by airway inflammation and airway hyperresponsiveness. Wogonoside, a flavonoid glycoside, is reported to significantly alleviate the inflammation response and oxidative stress. Herein, this study aimed to investigate the therapeutic effect and underlying mechanism of wogonoside on airway inflammation and mucus hypersecretion in a murine asthma model and in human bronchial epithelial cells (16HBE). BALB/c mice were sensitized and challenged with ovalbumin (OVA). Pulmonary function and the number of cells in the bronchoalveolar lavage fluid (BALF) were examined. Pathological changes in lung tissue in each group were evaluated via hematoxylin and eosin and periodic acid-Schiff staining, and changes in levels of cytokines in BALF and of immunoglobulin E in serum were determined via an enzyme-linked immunosorbent assay. The expression of relevant genes in lung tissue was analyzed via real-time PCR. Western blotting and immunofluorescence were employed to detect the expression of relevant proteins in lung tissue and 16HBE cells. Treatment with 10 and 20 mg/kg wogonoside significantly attenuated the OVA-induced increase of inflammatory cell infiltration, mucus secretion, and goblet cell percentage and improved pulmonary function. Wogonoside treatment reduced the level of T-helper 2 cytokines including interleukin (IL)-4, IL-5, and IL-13 in BALF and of IgE in serum and decreased the mRNA levels of cytokines (IL-4, IL-5, IL-6, IL-13, and IL-1 beta and tumor necrosis factor-alpha), chemokines (CCL-2, CCL-11, and CCL-24), and mucoproteins (MUC5AC, MUC5B, and GOB5) in lung tissues. The expression of MUC5AC and the phosphorylation of STAT6 and NF-kappa B p65 in lung tissues and 16HBE cells were significantly downregulated after wogonoside treatment. Thus, wogonoside treatment may effectively decrease airway inflammation, airway remodeling, and mucus hypersecretion via blocking NF-kappa B/STAT6 activation.

wogonoside asthma inflammation mucus hypersecretion NF-kappa B STAT6 MUC5AC

SCI

英语

第一 ; 通讯

National Natural Science Foundation of China["82100443","82170042","81770028"] ; National Natural Science Foundation of China["JCYJ20210324114400002","JCYJ20230807112201003"] ; Shenzhen Science and Technology Innovation Program[2022A1515111126]

Agriculture ; Chemistry ; Food Science & Technology

Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology

WOS:001189136200001

AMER CHEMICAL SOC

AGRICULTURAL SCIENCES

Web of Science

Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Shenzhen Inst Resp Dis, Dept Resp & Crit Care Med, Shenzhen 518020, Peoples R China

Yu, Xiu,Cai, Bicheng,Yu, Li,et al. Wogonoside Ameliorates Airway Inflammation and Mucus Hypersecretion via NF-κB/STAT6 Signaling in Ovalbumin-Induced Murine Acute Asthma[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2024,72(13).

Yu, Xiu.,Cai, Bicheng.,Yu, Li.,Li, Nan.,Wu, Chujie.,...&Qiu, Chen.(2024).Wogonoside Ameliorates Airway Inflammation and Mucus Hypersecretion via NF-κB/STAT6 Signaling in Ovalbumin-Induced Murine Acute Asthma.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,72(13).

Yu, Xiu,et al."Wogonoside Ameliorates Airway Inflammation and Mucus Hypersecretion via NF-κB/STAT6 Signaling in Ovalbumin-Induced Murine Acute Asthma".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72.13(2024).