南方科技大学知识苑(SUSTech KC): Vitamin D suppresses CD133+/CD44+cancer stem cell stemness by inhibiting NF-ÎºB signaling and reducing NLRP3 expression in triple-negative breast cancer

题名	Vitamin D suppresses CD133+/CD44+cancer stem cell stemness by inhibiting NF-ÎºB signaling and reducing NLRP3 expression in triple-negative breast cancer
作者	Zheng, Wei ; Peng, Wei ; Qian, Fuyong; Zhang, Mingshuai; Duan, Bofeng; Fan, Zhifeng; Xie, Yi; Fu, Xiaoying
通讯作者	Zheng, Wei
发表日期	2024-03-01
DOI	10.1007/s00280-024-04660-w
发表期刊	CANCER CHEMOTHERAPY AND PHARMACOLOGY 影响因子和分区
ISSN	0344-5704
EISSN	1432-0843
摘要	Background and objective This study aims to investigate the role of Vitamin D (VD) in regulating the stemness and survival of CD133+/CD44 + breast cancer stem cells, and to explore the role of NLRP3 in this process. Methods Breast cancer tissues were collected for RXR alpha and VDR expression analysis. A triple-negative breast cancer cell line was cultured and stem-like cells (CD133 + CD44+) isolated using flow cytometry. These cells were treated with VD, analyzing their stem-like properties, apoptosis and proliferation, as well as P65 nuclear expression and NLRP3 expression. After NLRP3 inflammasome activator treatment, the parameters were reassessed. RXR alpha and VDR interaction was confirmed using co-immunoprecipitation (CoIP). Finally, a subcutaneous xenograft model of triple-negative breast cancer was treated with VD and subsequently analyzed for stem-like properties, proliferation, apoptosis, and NLRP3 expression levels. ResultsCD133+/CD44 + stem cells expressed high levels of SOX2 and OCT4. VD treatment resulted in a significant decrease in SOX2 and OCT4 expression, fewer sphere-forming colonies, lower proliferation ability, and more apoptosis. Additionally, VD treatment inhibited NF-kappa B signaling and reduced NLRP3 expression. The NLRP3 activator BMS-986,299 counteracted the effects of VD in vitro. In vivo, VD inhibited the growth of breast cancer stem cells, reducing both tumor volume and weight, and decreased NLRP3, SOX2, and OCT4 expression within tumor tissues. Conclusion Findings elucidate that VD mediates the modulation of stemness in CD133+/CD44 + breast cancer stem cells through the regulation of NLRP3 expression. The research represents novel insights on the implications for the application of VD in cancer therapies.
关键词	Vitamin D RXR alpha Breast cancer stem cell NLRP3
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
WOS研究方向	Oncology ; Pharmacology & Pharmacy
WOS类目	Oncology ; Pharmacology & Pharmacy
WOS记录号	WOS:001177771300002
出版者	SPRINGER
ESI学科分类	CLINICAL MEDICINE
来源库	Web of Science
引用统计
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/788883
专题	南方科技大学第二附属医院
作者单位	Southern Univ Sci & Technol, Peoples Hosp Shenzhen 3, Dept Thyroid & Breast Surg, Hosp Affiliated 2, 29 Bulan Rd, Shenzhen 518112, Guangdong, Peoples R China
第一作者单位	南方科技大学第二附属医院
通讯作者单位	南方科技大学第二附属医院
第一作者的第一单位	南方科技大学第二附属医院
推荐引用方式 GB/T 7714	Zheng, Wei,Peng, Wei,Qian, Fuyong,et al. Vitamin D suppresses CD133+/CD44+cancer stem cell stemness by inhibiting NF-ÎºB signaling and reducing NLRP3 expression in triple-negative breast cancer[J]. CANCER CHEMOTHERAPY AND PHARMACOLOGY,2024.
APA	Zheng, Wei.,Peng, Wei.,Qian, Fuyong.,Zhang, Mingshuai.,Duan, Bofeng.,...&Fu, Xiaoying.(2024).Vitamin D suppresses CD133+/CD44+cancer stem cell stemness by inhibiting NF-ÎºB signaling and reducing NLRP3 expression in triple-negative breast cancer.CANCER CHEMOTHERAPY AND PHARMACOLOGY.
MLA	Zheng, Wei,et al."Vitamin D suppresses CD133+/CD44+cancer stem cell stemness by inhibiting NF-ÎºB signaling and reducing NLRP3 expression in triple-negative breast cancer".CANCER CHEMOTHERAPY AND PHARMACOLOGY (2024).