A converged ubiquitin-proteasome pathway for the degradation of TOC and TOM tail-anchored receptors

Yang, Meijing1,2; Chen, Shuai3,4; Lim, Shey-Li1; Yang, Lang1,2; Zhong, Jia Yi1; Chan, Koon Chuen1; Zhao, Zhizhu1; Wong, Kam-Bo4,5; Wang, Junqi2; Lim, Boon Leong1,5,6

2024-05-01

10.1111/jipb.13645

JOURNAL OF INTEGRATIVE PLANT BIOLOGY   影响因子和分区

1672-9072

1744-7909

In plants, thousands of nucleus-encoded proteins translated in the cytosol are sorted to chloroplasts and mitochondria by binding to specific receptors of the TOC (translocon on the outer chloroplast membrane) and the TOM (translocon on the outer mitochondrial membrane) complexes for import into those organelles. The degradation pathways for these receptors are unclear. Here, we discovered a converged ubiquitin-proteasome pathway for the degradation of Arabidopsis thaliana TOC and TOM tail-anchored receptors. The receptors are ubiquitinated by E3 ligase(s) and pulled from the outer membranes by the AAA+ adenosine triphosphatase CDC48, after which a previously uncharacterized cytosolic protein, transmembrane domain (TMD)-binding protein for tail-anchored outer membrane proteins (TTOP), binds to the exposed TMDs at the C termini of the receptors and CDC48, and delivers these complexes to the 26S proteasome.

26S proteasome CDC48 SP1 TOC TOM ubiquitination

SCI

英语

其他

Science, Technology and Innovation Commission of Shenzhen Municipality[201708183000803] ; Science, Technology and Innovation Commission of Shenzhen Municipality (Basic Research Program)[AoE/M-403/16]

Biochemistry & Molecular Biology ; Plant Sciences

Biochemistry & Molecular Biology ; Plant Sciences

WOS:001187043900001

WILEY

Web of Science

1.Univ Hong Kong, Sch Biol Sci, Hong Kong 999077, Peoples R China
2.Southern Univ Sci & Technol, Dept Biol, Shenzhen 518055, Peoples R China
3.Shenzhen Univ, Sch Biomed Engn, Shenzhen 518060, Peoples R China
4.Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong 999077, Peoples R China
5.Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Shatin, Hong Kong 999077, Peoples R China
6.HKU Shenzhen Inst Res & Innovat, Shenzhen, Peoples R China

Yang, Meijing,Chen, Shuai,Lim, Shey-Li,et al. A converged ubiquitin-proteasome pathway for the degradation of TOC and TOM tail-anchored receptors[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2024,66(5).

Yang, Meijing.,Chen, Shuai.,Lim, Shey-Li.,Yang, Lang.,Zhong, Jia Yi.,...&Lim, Boon Leong.(2024).A converged ubiquitin-proteasome pathway for the degradation of TOC and TOM tail-anchored receptors.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,66(5).

Yang, Meijing,et al."A converged ubiquitin-proteasome pathway for the degradation of TOC and TOM tail-anchored receptors".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 66.5(2024).