Leukemia inhibitory factor protects against experimental periodontitis through immuno-modulations of both macrophages and periodontal ligament fibroblasts

Ou, Yanjing1,2,3,4,5; Fan, Le6; Wang, Xiaoqi7; Xia, Haibin1; Cheng, Mengwen1; Huang, Jing1; Liang, Youde8; Wang, Yining1; Zhou, Yi1,9,10

2024-03-01

10.1002/JPER.23-0607

JOURNAL OF PERIODONTOLOGY   影响因子和分区

0022-3492

1943-3670

Background: To explore the role of leukemia inhibitory factor (LIF) in periodontitis via in vivo and in vitro experiments. Methods: The second upper molar of LIF knockout mice and their wild-type littermates were ligated for 8 days. Micro-computed tomography (micro-CT), histological analysis, and quantitative real-time polymerase chain reaction (qRT-PCR) were performed. The expression levels of proinflammatory cytokines were examined in mouse bone marrow derived macrophages and human periodontal ligament fibroblasts (HPDLFs) after lipopolysaccharide (LPS) treatment. Results: LIF deficiency promoted alveolar bone loss, inflammatory cells infiltration, osteoclasts formation and collagen fiber degradation in ligature-induced mouse, along with higher expressions of proinflammatory cytokines, including interleukin-6 (IL6), IL-1 beta (IL1B), tumor necrosis factor-alpha (TNFA), matrix metalloproteinase 13 (MMP13), and RANKL/OPG ratio. Additionally, LIF deletion led to higher expression levels of these proinflammatory cytokines in mouse bone marrow-derived macrophages from both femur and alveolar bone and HPDLFs when treated with LPS. Administration of recombined LIF attenuated TNFA, IL1B, and RANKL/OPG ratio in HPDLFs. Conclusions: These findings indicate that LIF deficiency promotes the progress of periodontitis via modulating immuno-inflammatory responses of macrophages and periodontal ligament fibroblasts, and the application of LIF may be an adjunctive treatment for periodontitis to resolute inflammation.

leukemia inhibitory factor lipopolysaccharide macrophage osteoclast periodontitis

SCI

英语

其他

Wuhan Science and Technology Project[2016060101010043] ; Natural Science Foundation of Fujian Province[2022J01267] ; Fujian Provincial Health Technology Project[2021GGA052] ; null[82071090]

Dentistry, Oral Surgery & Medicine

Dentistry, Oral Surgery & Medicine

WOS:001185547800001

WILEY

CLINICAL MEDICINE

Web of Science

1.Wuhan Univ, State Key Lab Oral & Maxillofacial Reconstruct & R, Hubei Key Lab Stomatol, Key Lab Oral Biomed,Minist Educ,Sch & Hosp Stomato, Wuhan, Hubei, Peoples R China
2.Fujian Med Univ, Sch & Hosp Stomatol, Postdoctoral Workstat, Fuzhou, Peoples R China
3.Fujian Med Univ, Sch & Hosp Stomatol, Fujian Key Lab Oral Dis, Fuzhou, Peoples R China
4.Fujian Med Univ, Sch & Hosp Stomatol, Fujian Prov Engn Res Ctr Oral Biomat, Fuzhou, Peoples R China
5.Fujian Med Univ, Stomatol Key Lab Fujian Coll & Univ, Sch & Hosp Stomatol, Fuzhou, Peoples R China
6.Sun Yat Sen Univ, Hosp Stomatol, Guanghua Sch Stomatol, Dept Prosthodont, Guangzhou, Guangdong, Peoples R China
7.Soochow Univ, Affiliated Hosp 1, Dept Stomatol, Suzhou, Jiangsu, Peoples R China
8.Southern Univ Sci & Technol, Yantian Hosp, Dept Stomatol, Shenzhen, Guangdong, Peoples R China
9.Wuhan Univ, Sch & Hosp Stomatol, State Key Lab Breeding Base Basic Sci Stomatol Hub, Minist Educ, 237 Luoyu Rd, Wuhan 430079, Peoples R China
10.Wuhan Univ, Sch & Hosp Stomatol, Key Lab Oral Biomed, Minist Educ, 237 Luoyu Rd, Wuhan 430079, Peoples R China

Ou, Yanjing,Fan, Le,Wang, Xiaoqi,et al. Leukemia inhibitory factor protects against experimental periodontitis through immuno-modulations of both macrophages and periodontal ligament fibroblasts[J]. JOURNAL OF PERIODONTOLOGY,2024.

Ou, Yanjing.,Fan, Le.,Wang, Xiaoqi.,Xia, Haibin.,Cheng, Mengwen.,...&Zhou, Yi.(2024).Leukemia inhibitory factor protects against experimental periodontitis through immuno-modulations of both macrophages and periodontal ligament fibroblasts.JOURNAL OF PERIODONTOLOGY.

Ou, Yanjing,et al."Leukemia inhibitory factor protects against experimental periodontitis through immuno-modulations of both macrophages and periodontal ligament fibroblasts".JOURNAL OF PERIODONTOLOGY (2024).