Recent advances of single-cell RNA sequencing in toxicology research: Insight into hepatotoxicity and nephrotoxicity

Chen, Junhui1; Wu, Jiangpeng1; Bai, Yunmeng1; Yang, Chuanbin1; Wang, Jigang1,2,3

2024-03-01

10.1016/j.cotox.2024.100462

CURRENT OPINION IN TOXICOLOGY   影响因子和分区

2468-2020

The advanced single -cell RNA sequencing (scRNA-seq) technology enables the measurement of gene expression levels and identification of cell -specific markers in individual cells, and the revealing of cell types, providing valuable insights into cell behavior. ScRNA-seq has emerged as an effective method for detecting rare cell populations, cellular heterogeneity, and cell -to -cell crosstalk under physiology and pathology conditions, thereby advancing the revolution of toxicology research. In this review, we first briefly introduce the concept of hepatotoxicity and nephrotoxicity, along with their underlying mechanisms. We then summarize the singlecell sequencing technology, with an emphasis on single -cell isolation techniques, particularly for the most used dropletbased methods. Finally, we review the recent advances in the application of single -cell RNA sequencing in studying hepatotoxicity and nephrotoxicity. Overall, this review provides a comprehensive understanding of the recent advances in hepatotoxicity and nephrotoxicity studies at a single -cell resolution.

Hepatotoxicity Nephrotoxicity scRNA sequencing Application of scRNA sequencing.

ESCI

英语

第一 ; 通讯

Shenzhen Science and Technology Innovation Commission["JCYJ20210324114014039","JCYJ20210324115800001","JCYJ20220818102613029"] ; Shenzhen Medical Research Fund[B2302051] ; Shenzhen Governmental Sustainable Development Fund[KCXFZ20201221173612034] ; National Natural Science Foundation of China[82374063] ; Shenzhen key Laboratory of Kidney Diseases[ZDSYS201504301616234] ; Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties[SZGSP001]

Toxicology

Toxicology

WOS:001174028700001

ELSEVIER

Web of Science

1.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Shenzhen Clin Res Ctr Geriatr, Shenzhen 518020, Peoples R China
2.China Acad Chinese Med Sci, Artemisinin Res Ctr, State Key Lab Qual Assurance & Sustainable Use Dao, Beijing 100700, Peoples R China
3.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China

Chen, Junhui,Wu, Jiangpeng,Bai, Yunmeng,et al. Recent advances of single-cell RNA sequencing in toxicology research: Insight into hepatotoxicity and nephrotoxicity[J]. CURRENT OPINION IN TOXICOLOGY,2024,37.

Chen, Junhui,Wu, Jiangpeng,Bai, Yunmeng,Yang, Chuanbin,&Wang, Jigang.(2024).Recent advances of single-cell RNA sequencing in toxicology research: Insight into hepatotoxicity and nephrotoxicity.CURRENT OPINION IN TOXICOLOGY,37.

Chen, Junhui,et al."Recent advances of single-cell RNA sequencing in toxicology research: Insight into hepatotoxicity and nephrotoxicity".CURRENT OPINION IN TOXICOLOGY 37(2024).