Photoswitchable Temperature Nanosensors Based on the Chemical Kinetics of Photochromic Naphthopyran for Live Cell Imaging

Cheng, Yu1; Wu, Jianhong2; Cui, Yunxin1; Zhai, Jingying2; Wu, Minghui1; Xie, Xiaojiang1

2024-03-08

10.1021/acs.analchem.3c05568

ANALYTICAL CHEMISTRY   影响因子和分区

0003-2700

1520-6882

Microscopic temperature imaging holds significant importance in various fields, particularly in the development of nanomaterials for photothermal therapy (PTT). In this study, we present an analytical method to probe cellular temperature based on chemical kinetics and additional luminescence quenching by photoswitchable naphthopyrans. Taking advantage of the rapid ring-closing reaction of naphthopyran, temperature sensing was realized with a linear relationship between the logarithmic decay time constant (ln tau) and the reciprocal temperature (T (-1)). To create luminescent temperature nanosensors, we harnessed the ability of ring-opened naphthopyran to quench the luminescence of a semiconducting polymer, resulting in a diverse array of probes. Structural modifications on the naphthopyran also provided a way to fine-tune the sensitivity and response window of the nanosensors. The method allowed cellular temperature imaging on a cost-effective fluorescence microscopic setup. As an application, the temperature increase induced by gold nanorods (AuNRs) in cell lysosomes was successfully monitored, laying the foundation for a new class of photoswitchable nanosensors with promising biological applications.

SCI ; EI

英语

第一 ; 通讯

Guangdong Science and Technology Innovation Foundation["22274070","22304071"] ; National Natural Science Foundation of China[202110293000007]

Chemistry

Chemistry, Analytical

WOS:001181862400001

AMER CHEMICAL SOC

CHEMISTRY

Web of Science

1.Southern Univ Sci & Technol, Dept Chem, Shenzhen 518055, Peoples R China
2.Southern Univ Sci & Technol, Acad Adv Interdisciplinary Studies, Shenzhen 518055, Peoples R China

Cheng, Yu,Wu, Jianhong,Cui, Yunxin,et al. Photoswitchable Temperature Nanosensors Based on the Chemical Kinetics of Photochromic Naphthopyran for Live Cell Imaging[J]. ANALYTICAL CHEMISTRY,2024,96(11).

Cheng, Yu,Wu, Jianhong,Cui, Yunxin,Zhai, Jingying,Wu, Minghui,&Xie, Xiaojiang.(2024).Photoswitchable Temperature Nanosensors Based on the Chemical Kinetics of Photochromic Naphthopyran for Live Cell Imaging.ANALYTICAL CHEMISTRY,96(11).

Cheng, Yu,et al."Photoswitchable Temperature Nanosensors Based on the Chemical Kinetics of Photochromic Naphthopyran for Live Cell Imaging".ANALYTICAL CHEMISTRY 96.11(2024).