南方科技大学知识苑(SUSTech KC): Aqueous alkaline phosphate facilitates the non-exchangeable deuteration of peptides and proteins

题名	Aqueous alkaline phosphate facilitates the non-exchangeable deuteration of peptides and proteins
作者	Zhang, Tingting 1,2; Jin, Zhixiong 1,3; Zhao, Heng 1; Lai, Can 1,2; Liu, Zheyi 1; Luo, Pan 1,4; Dong, Zhe5,6 ; Wang, Fangjun 1,2
通讯作者	Wang, Fangjun
发表日期	2024-03-06
DOI	10.1039/d3ra08636d
发表期刊	RSC ADVANCES 影响因子和分区
EISSN	2046-2069
卷号	14 期号:12
摘要	["The incorporation of deuterium into peptides and proteins holds broad applications across various fields, such as drug development and structural characterization. Nevertheless, current methods for peptide/protein deuteration often target exchangeable labile sites or require harsh conditions for stable modification. In this study, we present a late-stage approach utilizing an alkaline phosphate solution to achieve deuteration of non-exchangeable backbone sites of peptides and proteins. The specific deuteration regions are identified through ultraviolet photodissociation (UVPD) and mass spectrometry analysis. This deuteration strategy demonstrates site and structure selectivity, with a notable affinity for labeling the alpha-helix regions of myoglobin. The deuterium method is particularly suitable for peptides and proteins that remain stable under high pH conditions.","The incorporation of deuterium into peptides and proteins holds broad applications across various fields, such as drug development and structural characterization."]
相关链接	[来源记录]
收录类别	SCI ; EI
语种	英语
学校署名	其他
资助项目	National Natural Science Foundation of China["22288201","22204165"] ; National Natural Science Foundation of China["DICP I202007","DICP I202228"]
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:001180685900001
出版者	ROYAL SOC CHEMISTRY
来源库	Web of Science
引用统计
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/788990
专题	深圳格拉布斯研究院理学院_化学系
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Zhejiang Univ, Dept Chem, Hangzhou 310027, Peoples R China 4.Inst Adv Sci Facil, Shenzhen 518000, Peoples R China 5.Southern Univ Sci & Technol, Shenzhen Grubbs Inst, Shenzhen 518055, Peoples R China 6.Southern Univ Sci & Technol, Dept Chem, Guangdong Prov Key Lab Catalysis, Shenzhen 518000, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Tingting,Jin, Zhixiong,Zhao, Heng,et al. Aqueous alkaline phosphate facilitates the non-exchangeable deuteration of peptides and proteins[J]. RSC ADVANCES,2024,14(12).
APA	Zhang, Tingting.,Jin, Zhixiong.,Zhao, Heng.,Lai, Can.,Liu, Zheyi.,...&Wang, Fangjun.(2024).Aqueous alkaline phosphate facilitates the non-exchangeable deuteration of peptides and proteins.RSC ADVANCES,14(12).
MLA	Zhang, Tingting,et al."Aqueous alkaline phosphate facilitates the non-exchangeable deuteration of peptides and proteins".RSC ADVANCES 14.12(2024).