A sensitive lateral flow test strip sensor for visual detection of acid red 18 in food using bicentric-emission carbon dots

Hu, Houwen1; Chen, Zewei2; Li, Tingting1; Wang, Linfan1; Xing, Haoming1; Guo, Guoqiang1,3; Wang, Gang1,4; Chen, Da1

2024-03-14

10.1039/d3nr05662g

NANOSCALE   影响因子和分区

2040-3364

2040-3372

["Hazardous synthetic colorants have found widespread use in food production, and excessive consumption of these pigments can pose potential risks to human health. In this study, we propose an ultrasensitive fluorescence method for the analysis of Acid Red 18 (AR18) in food products. The method is based on the nitrogen-doped carbon dots (N-CDs) derived from tris and resorcinol through a hydrothermal way. The as-synthesized N-CDs exhibit two emission centers at 425 nm and 541 nm, corresponding to the excitation wavelengths of 377 nm and 465 nm, respectively. Upon the addition of AR18, the fluorescence intensity at 541 nm significantly decreases with a simultaneous, though less pronounced, reduction in the intensity at 425 nm, which is attributed to the localization of fluorescence resonance energy transfer (L-FRET). Specifically, a novel ratiometric fluorescent probe was constructed based on the extracted data from the 3D fluorescence excitation-emission matrix. This probe demonstrates a wide linear range from 0.0539 to 30 mu M and a low limit of detection (LOD) of 53.9 nM. For practical applications, a portable fluorescent sensor based on a lateral flow test strip (LFTS) was designed for real-time monitoring of AR18. Color channel values were determined using a smartphone application, resulting in a satisfactory LOD of 75.3 nM. Furthermore, the suitability of the proposed ratiometric fluorescent probe was validated through the detection of AR18 in real food samples, consistently achieving recovery rates in the range of 99.7-101.4%. This research not only expands the scope of CDs in sensing fields, but also provides an effective strategy for the development of an excellent platform for real-time AR18 detection, contributing to public food safety.","Hazardous synthetic colorants have found widespread use in food production, and excessive consumption of these pigments can pose potential risks to human health."]

SCI ; EI

英语

其他

National Natural Science Foundation of China["61604084","62174093"] ; K. C. Wong Magna Fund in Ningbo University[2023QL006] ; Ningbo Youth Science and Technology Innovation Leading Talent Project[NKLJC-K2023-01]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied

WOS:001171943300001

ROYAL SOC CHEMISTRY

Web of Science

1.Ningbo Univ, Sch Phys Sci & Technol, Dept Microelect Sci & Engn, Ningbo 315211, Peoples R China
2.Saga Univ, Synchrotron Light Applicat Ctr, Dept Elect & Elect Engn, Saga 8408502, Japan
3.Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen Key Lab Full Spectral Solar Elect Generat, Shenzhen 518055, Peoples R China
4.Shanghai Inst Microsyst & Informat Technol, Chinese Acad Sci, Natl Key Lab Mat Integrated Circuits, Shanghai 200050, Peoples R China

Hu, Houwen,Chen, Zewei,Li, Tingting,et al. A sensitive lateral flow test strip sensor for visual detection of acid red 18 in food using bicentric-emission carbon dots[J]. NANOSCALE,2024,16(11).

Hu, Houwen.,Chen, Zewei.,Li, Tingting.,Wang, Linfan.,Xing, Haoming.,...&Chen, Da.(2024).A sensitive lateral flow test strip sensor for visual detection of acid red 18 in food using bicentric-emission carbon dots.NANOSCALE,16(11).

Hu, Houwen,et al."A sensitive lateral flow test strip sensor for visual detection of acid red 18 in food using bicentric-emission carbon dots".NANOSCALE 16.11(2024).