Uncovering the Bronchoalveolar Single-Cell Landscape of Patients With Pulmonary Tuberculosis With Human Immunodeficiency Virus Type 1 Coinfection

Xiao, Guohui1; Huang, Waidong2,3; Zhong, Yu2; Ou, Min1; Ye, Taosheng1; Wang, Zhifeng2; Zou, Xuanxuan2,3; Ding, Feng1; Yang, Yuan2; Zhang, Zhe4; Liu, Chuanyu2,5; Liu, Aimei6; Liu, Longqi2,5; Lu, Shuihua1; Wu, Liang2,7,9; Zhang, Guoliang1,8

2024-02-01

10.1093/infdis/jiae042

JOURNAL OF INFECTIOUS DISEASES   影响因子和分区

0022-1899

1537-6613

["Background Coinfection of human immunodeficiency virus type 1 (HIV-1) is the most significant risk factor for tuberculosis (TB). The immune responses of the lung are essential to restrict the growth of Mycobacterium tuberculosis and avoid the emergence of the disease. Nevertheless, there is still limited knowledge about the local immune response in people with HIV-1-TB coinfection.Methods We employed single-cell RNA sequencing (scRNA-seq) on bronchoalveolar lavage fluid from 9 individuals with HIV-1-TB coinfection and 10 with pulmonary TB.Results A total of 19 058 cells were grouped into 4 major cell types: myeloid cells, T/natural killer (NK) cells, B cells, and epithelial cells. The myeloid cells and T/NK cells were further divided into 10 and 11 subsets, respectively. The proportions of dendritic cell subsets, CD4+ T cells, and NK cells were lower in the HIV-1-TB coinfection group compared to the TB group, while the frequency of CD8+ T cells was higher. Additionally, we identified numerous differentially expressed genes between the CD4+ and CD8+ T-cell subsets between the 2 groups.Conclusions HIV-1 infection not only affects the abundance of immune cells in the lungs but also alters their functions in patients with pulmonary TB.","We utilized single-cell RNA sequencing technology to provide a detailed analysis of immune cells in the lungs of patients with tuberculosis (TB) or with HIV-TB coinfection. Our findings revealed notable variations in immune response between these 2 disease types."]

single-cell RNA sequencing pulmonary TB HIV-1 bronchoalveolar lavage fluid local immunity

SCI

英语

第一 ; 通讯

National Key Research and Development Plan[2021YFA1300902] ; National Natural Science Foundation of China[82170009] ; Guangdong Science Fund for Distinguished Young Scholars[0620220214] ; State Key Laboratory of Respiratory Diseases Open Project[SKLRD-OP-202324] ; Shenzhen Scientific and Technological Foundation["KCXFZ20211020163545004","RCJC20221008092726022","LCYX20220620105200001"] ; Guangxi Scientific and Technological Foundation[AA22096027] ; Shenzhen Key Laboratory of Single-Cell Omics[ZDSYS20190902093613831]

Immunology ; Infectious Diseases ; Microbiology

Immunology ; Infectious Diseases ; Microbiology

WOS:001173806000001

OXFORD UNIV PRESS INC

IMMUNOLOGY

Web of Science

1.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, Shenzhen, Peoples R China
2.BGI Res, Shenzhen, Peoples R China
3.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
4.MGI Tech, Shenzhen, Peoples R China
5.BGI Res, Hangzhou, Peoples R China
6.Guangxi Chest Hosp, Dept TB, Liuzhou, Peoples R China
7.BGI Res, Chongqing, Peoples R China
8.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, Bulan Rd, Shenzhen 518112, Peoples R China
9.BGI Res, Beishan Rd, Shenzhen, Peoples R China

Xiao, Guohui,Huang, Waidong,Zhong, Yu,et al. Uncovering the Bronchoalveolar Single-Cell Landscape of Patients With Pulmonary Tuberculosis With Human Immunodeficiency Virus Type 1 Coinfection[J]. JOURNAL OF INFECTIOUS DISEASES,2024.

Xiao, Guohui.,Huang, Waidong.,Zhong, Yu.,Ou, Min.,Ye, Taosheng.,...&Zhang, Guoliang.(2024).Uncovering the Bronchoalveolar Single-Cell Landscape of Patients With Pulmonary Tuberculosis With Human Immunodeficiency Virus Type 1 Coinfection.JOURNAL OF INFECTIOUS DISEASES.

Xiao, Guohui,et al."Uncovering the Bronchoalveolar Single-Cell Landscape of Patients With Pulmonary Tuberculosis With Human Immunodeficiency Virus Type 1 Coinfection".JOURNAL OF INFECTIOUS DISEASES (2024).