NIR-II Conjugated Electrolytes as Biomimetics of Lipid Bilayers for In Vivo Liposome Tracking

Meng, Yingying1; Gao, Ji2; Zhou, Peirong1; Qin, Xudong1; Tian, Miao3; Wang, Xiaohui3; Zhou, Cheng1; Li, Kai2; Huang, Fei1; Cao, Yong1

2024-03-22

10.1002/anie.202318632

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   影响因子和分区

1433-7851

1521-3773

Liposomes serve as promising and versatile vehicles for drug delivery. Tracking these nanosized vesicles, particularly in vivo, is crucial for understanding their pharmacokinetics. This study introduces the design and synthesis of three new conjugated electrolyte (CE) molecules, which emit in the second near-infrared window (NIR-II), facilitating deeper tissue penetration. Additionally, these CEs, acting as biomimetics of lipid bilayers, demonstrate superior compatibility with lipid membranes compared to commonly used carbocyanine dyes like DiR. To counteract the aggregation-caused quenching effect, CEs employ a twisted backbone, as such their fluorescence intensities can effectively enhance after a fluorophore multimerization strategy. Notably, a "passive" method was employed to integrate CEs into liposomes during the liposome formation, and membrane incorporation efficiency was significantly promoted to nearly 100%. To validate the in vivo tracking capability, the CE-containing liposomes were functionalized with cyclic arginine-glycine-aspartic acid (cRGD) peptides, serving as tumor-targeting ligands. Clear fluorescent images visualizing tumor site in living mice were captured by collecting the NIR-II emission. Uniquely, these CEs exhibit additional emission peak in visible region, enabling in vitro subcellular analysis using routine confocal microscopy. These results underscore the potential of CEs as a new-generation of membrane-targeting probes to facilitate the liposome-based medicine research.

Conjugated Electrolyte NIR-II Liposome Labeling Lipid Bilayers Dual-emission

SCI ; EI

英语

通讯

National Natural Science Foundation of China[U21A2097] ; Shenzhen Science and Technology Program["JSGG20200225151916021","SUSTech-JY202202002"] ; null[2019B030302007]

Chemistry

Chemistry, Multidisciplinary

WOS:001172847600001

WILEY-V C H VERLAG GMBH

CHEMISTRY

Web of Science

1.South China Univ Technol, Inst Polymer Optoelect Mat & Devices, Guangdong Basic Res Ctr Excellence Energy & Inform, Sch Mat Sci & Engn,State Key Lab Luminescent Mat &, Guangzhou 510640, Peoples R China
2.Southern Univ Sci & Technol, Dept Biomed Engn, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Peoples R China
3.South China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Peoples R China

Meng, Yingying,Gao, Ji,Zhou, Peirong,et al. NIR-II Conjugated Electrolytes as Biomimetics of Lipid Bilayers for In Vivo Liposome Tracking[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2024,63(13).

Meng, Yingying.,Gao, Ji.,Zhou, Peirong.,Qin, Xudong.,Tian, Miao.,...&Cao, Yong.(2024).NIR-II Conjugated Electrolytes as Biomimetics of Lipid Bilayers for In Vivo Liposome Tracking.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,63(13).

Meng, Yingying,et al."NIR-II Conjugated Electrolytes as Biomimetics of Lipid Bilayers for In Vivo Liposome Tracking".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 63.13(2024).