Light-Field Microscopy-Based Counting Of Particles Moving in a Microfluidic Volume

Xinglong Huang1,2; Xing Cheng2; Boris Stoeber1

2023-06-29

2167-0013

979-8-3503-3302-2

2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers)

25-29 June 2023

Kyoto, Japan

This paper presents a three-dimensional (3D) imaging flow cytometry (IFC) based on light-field microscopy (LFM) that is capable to capture 3D spatial information of an entire microfluidic volume by a single exposure. Our LFM 3D IFC uses a custom microlens array (MLA) that is fabricated with a low-cost method and achieves on-chip continuous 3D imaging of particles flowing in a microfluidic channel. Our counting algorithm uses a sequential intensity peak search to identify the locations of the individual particles, and it has a counting accuracy of ~92.6%. Compared with other 3D IFC, our device can achieve a higher throughput, and it avoids false counts due to particle occlusion.

其他

1.The University of British Columbia, Canada
2.Southern University of Science and Technology, China

Xinglong Huang,Xing Cheng,Boris Stoeber. Light-Field Microscopy-Based Counting Of Particles Moving in a Microfluidic Volume[C],2023.