FLOT2 promotes nasopharyngeal carcinoma progression through suppression of TGF-b pathway via facilitating CD109 expression

Xu, Hongjuan1,2,3; Yin, Yuze1,2,4; Li, Yihan1,2,4; Shi, Ning1,2,4; Xie, Wen1,2,4; Luo, Weiren5; Wang, Lei1,2,4; Zhu, Bin1,2,4; Liu, Weidong1,2,4; Jiang, Xingjun2,6; Ren, Caiping1,2,4

2024-01-19

10.1016/j.isci.2023.108580

ISCIENCE   影响因子和分区

2589-0042

In nasopharyngeal carcinoma (NPC), the TGF-b/Smad pathway genes are altered with inactive TGF-b signal, but the mechanisms remain unclear. RNA-sequencing results showed that FLOT2 negatively regulated the TGF-b signaling pathway via up-regulating CD109 expression. qRT-PCR, western blot, ChIP, and dual-luciferase assays were used to identify whether STAT3 is the activating transcription factor of CD109. Co-IP immunofluorescence staining assays were used to demonstrate the connection between FLOT2 and STAT3. In vitro and in vivo experiments were used to detect whether CD109 could rescue the functional changes of NPC cells resulting from FLOT2 alteration. IHC and Spearman correlation coefficients were used to assay the correlation between FLOT2 and CD109 expression in NPC tissues. Our results found that FLOT2 promotes the development of NPC by inhibiting TGF-b signaling pathway via stimulating the expression of CD109 by stabilizing STAT3, which provides a potential therapeutic strategy for NPC treatment.

SCI

英语

其他

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:001160779900001

CELL PRESS

Web of Science

1.Cent South Univ, Xiangya Hosp, Dept Neurosurg, NHC Key Lab Carcinogenesis,NHC Key Lab Nanobiol Te, Changsha, Hunan, Peoples R China
2.Cent South Univ, Xiangya Hosp, Natl Clin Res Ctr Geriatr Disorders, Changsha, Hunan, Peoples R China
3.Cent South Univ, Xiangya Hosp, PET Ctr, Nucl Med, Changsha, Hunan, Peoples R China
4.Cent South Univ, Canc Res Inst Sch Basic Med Sci, Chinese Minist Educ, Key Lab Carcinogenesis & Canc Invas, Changsha, Hunan, Peoples R China
5.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Affiliated Hosp 2, Canc Res Inst, Shenzhen, Guangdong, Peoples R China
6.Cent South Univ, Xiangya Hosp, Dept Neurosurg, Changsha, Peoples R China

Xu, Hongjuan,Yin, Yuze,Li, Yihan,et al. FLOT2 promotes nasopharyngeal carcinoma progression through suppression of TGF-b pathway via facilitating CD109 expression[J]. ISCIENCE,2024,27(1).

Xu, Hongjuan.,Yin, Yuze.,Li, Yihan.,Shi, Ning.,Xie, Wen.,...&Ren, Caiping.(2024).FLOT2 promotes nasopharyngeal carcinoma progression through suppression of TGF-b pathway via facilitating CD109 expression.ISCIENCE,27(1).

Xu, Hongjuan,et al."FLOT2 promotes nasopharyngeal carcinoma progression through suppression of TGF-b pathway via facilitating CD109 expression".ISCIENCE 27.1(2024).