Phage Display Screening of Anchor Peptides for Red Blood Cell-Derived Extracellular Vesicles

Xu, Limei1,2; Xu, Xiao1,2; Xia, Jiang3; Zhang, Huawei4,5; Liang, Yujie6; Duan, Li1

2024-01-31

10.1021/acsomega.3c06527

ACS OMEGA   影响因子和分区

2470-1343

Extracellular vesicles (EVs) are increasingly used for disease diagnosis and treatment. Among them, red blood cell-derived EVs (RBC-EVs) have attracted great attention due to their abundant sources and low risks of gene transfer (RBC-EVs lack nuclear and mitochondrial DNA). Here, we first revealed the high expression level of membrane protein solute carrier family 4 member 1 (SLC4A1) in RBC-EVs through proteomic analysis. We then identified several binding peptides with high affinity for the SLC4A1 extracellular domain (SLC4A1-EC) from phage display library screening. A high affinity of SLC4A1-EC and the three peptides (XRB2, XRE4, and XRH7) were assessed in vitro using surface plasmon resonance analysis and SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The binding sites of SLC4A1-EC and polypeptides were further predicted by LigPlot + analysis, and the results showed that these three polypeptides could bind to part of the hydrophobic residues of SLC4A1-EC. The binding efficiency of the anchor peptides to the RBC-EVs was further verified by flow cytometry and fluorescence imaging. In conclusion, we successfully screened three specific RBC-EV-targeting peptides which could potentially be utilized for isolating RBC-derived EVs from serum samples. More importantly, this peptide could be coupled with targeting peptides to modify RBC-EVs for drug delivery. Our work will provide a viable method for optimizing the function of RBC-EVs.

SCI

英语

其他

National Natural Science Foundation of China["81972116","81772394","82372269","31900046"] ; Shenzhen Science and Technology Projects["SGDX20201103095800003","GJHZ20200731095606019","JCYJ20170817172023838"] ; Guangdong International Cooperation Project[2021A0505030011] ; Shenzhen Fund for Guangdong Provincial High Level Clinical Key Specialties[SZGSP013] ; Shenzhen Key Medical Discipline Construction Fund[SZXK042] ; Medical-Engineering Interdisciplinary Research Foundation of Shenzhen University[2023YG014] ; Shandong Provincial Natural Science Foundation[ZR2023QH148] ; PhDResearch Foundation of Affiliated Hospital of Jining Medical University[2022-BS-04]

Chemistry

Chemistry, Multidisciplinary

WOS:001158991000001

AMER CHEMICAL SOC

Web of Science

1.Shenzhen Univ, Shenzhen Peoples Hosp 2, Guangdong Prov Res Ctr Artificial Intelligence & D, Dept Orthoped,Affiliated Hosp 1, Shenzhen 518035, Guangdong, Peoples R China
2.Jining Med Univ, Affiliated Hosp, Jining 272029, Shandong, Peoples R China
3.Chinese Univ Hong Kong, Dept Chem, Shatin, Hong Kong 999077, Peoples R China
4.Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen 518055, Guangdong, Peoples R China
5.South Univ Sci & Technol China, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
6.ShenzhenKangning Hosp, Shenzhen Inst Mental Hlth, Shenzhen Mental Hlth Ctr, Dept Child & Adolescent Psychiat, Shenzhen 518020, Guangdong, Peoples R China

Xu, Limei,Xu, Xiao,Xia, Jiang,et al. Phage Display Screening of Anchor Peptides for Red Blood Cell-Derived Extracellular Vesicles[J]. ACS OMEGA,2024,9(6).

Xu, Limei,Xu, Xiao,Xia, Jiang,Zhang, Huawei,Liang, Yujie,&Duan, Li.(2024).Phage Display Screening of Anchor Peptides for Red Blood Cell-Derived Extracellular Vesicles.ACS OMEGA,9(6).

Xu, Limei,et al."Phage Display Screening of Anchor Peptides for Red Blood Cell-Derived Extracellular Vesicles".ACS OMEGA 9.6(2024).