Association of the pathomics-collagen signature with lymph node metastasis in colorectal cancer: a retrospective multicenter study

Jiang, Wei1,2; Wang, Huaiming3,4,5; Dong, Xiaoyu1; Zhao, Yandong6; Long, Chenyan1,7; Chen, Dexin1; Yan, Botao1; Cheng, Jiaxin1; Lin, Zexi2; Zhuo, Shuangmu2; Wang, Hui3; Yan, Jun1,8

2024-01-25

10.1186/s12967-024-04851-2

JOURNAL OF TRANSLATIONAL MEDICINE   影响因子和分区

1479-5876

Background Lymph node metastasis (LNM) is a prognostic biomarker and affects therapeutic selection in colorectal cancer (CRC). Current evaluation methods are not adequate for estimating LNM in CRC. H&E images contain much pathological information, and collagen also affects the biological behavior of tumor cells. Hence, the objective of the study is to investigate whether a fully quantitative pathomics-collagen signature (PCS) in the tumor microenvironment can be used to predict LNM. Methods Patients with histologically confirmed stage I-III CRC who underwent radical surgery were included in the training cohort (n = 329), the internal validation cohort (n = 329), and the external validation cohort (n = 315). Fully quantitative pathomics features and collagen features were extracted from digital H&E images and multiphoton images of specimens, respectively. LASSO regression was utilized to develop the PCS. Then, a PCS-nomogram was constructed incorporating the PCS and clinicopathological predictors for estimating LNM in the training cohort. The performance of the PCS-nomogram was evaluated via calibration, discrimination, and clinical usefulness. Furthermore, the PCS-nomogram was tested in internal and external validation cohorts. Results By LASSO regression, the PCS was developed based on 11 pathomics and 9 collagen features. A significant association was found between the PCS and LNM in the three cohorts (P < 0.001). Then, the PCS-nomogram based on PCS, preoperative CEA level, lymphadenectasis on CT, venous emboli and/or lymphatic invasion and/or perineural invasion (VELIPI), and pT stage achieved AUROCs of 0.939, 0.895, and 0.893 in the three cohorts. The calibration curves identified good agreement between the nomogram-predicted and actual outcomes. Decision curve analysis indicated that the PCS-nomogram was clinically useful. Moreover, the PCS was still an independent predictor of LNM at station Nos. 1, 2, and 3. The PCS nomogram displayed AUROCs of 0.849-0.939 for the training cohort, 0.837-0.902 for the internal validation cohort, and 0.851-0.895 for the external validation cohorts in the three nodal stations. Conclusions This study proposed that PCS integrating pathomics and collagen features was significantly associated with LNM, and the PCS-nomogram has the potential to be a useful tool for predicting individual LNM in CRC patients.

Colorectal cancer Lymph node metastasis Pathomics Collagen features

SCI

英语

通讯

Research & Experimental Medicine

Medicine, Research & Experimental

WOS:001151370900002

BMC

Web of Science

1.Southern Med Univ, Nanfang Hosp, Sch Clin Med 1, Dept Gen Surg,Guangdong Prov Key Lab Precis Med Ga, Guangzhou 510515, Guangdong, Peoples R China
2.Jimei Univ, Sch Sci, Xiamen 361021, Fujian, Peoples R China
3.Sun Yat Sen Univ, Affiliated Hosp 6, Dept Gen Surg Colorectal Surg, Guangzhou 510655, Guangdong, Peoples R China
4.Sun Yat Sen Univ, Affiliated Hosp 6, Guangdong Prov Key Lab Colorectal & Pelv Floor Dis, Guangzhou 510655, Guangdong, Peoples R China
5.Sun Yat Sen Univ, Affiliated Hosp 6, Biomed Innovat Ctr, Guangzhou 510655, Guangdong, Peoples R China
6.Sun Yat Sen Univ, Affiliated Hosp 6, Dept Pathol, Guangzhou 510655, Guangdong, Peoples R China
7.Guangxi Med Univ, Canc Hosp, Dept Gastrointestinal Surg, Div Colorectal & Anal Surg, Nanning, Peoples R China
8.Southern Univ Sci & Technol, Jinan Univ, Affiliated Hosp 1, Dept Gastrointestinal Surg,Shenzhen Peoples Hosp,C, Shenzhen 518020, Guangdong, Peoples R China

Jiang, Wei,Wang, Huaiming,Dong, Xiaoyu,et al. Association of the pathomics-collagen signature with lymph node metastasis in colorectal cancer: a retrospective multicenter study[J]. JOURNAL OF TRANSLATIONAL MEDICINE,2024,22(1).

Jiang, Wei.,Wang, Huaiming.,Dong, Xiaoyu.,Zhao, Yandong.,Long, Chenyan.,...&Yan, Jun.(2024).Association of the pathomics-collagen signature with lymph node metastasis in colorectal cancer: a retrospective multicenter study.JOURNAL OF TRANSLATIONAL MEDICINE,22(1).

Jiang, Wei,et al."Association of the pathomics-collagen signature with lymph node metastasis in colorectal cancer: a retrospective multicenter study".JOURNAL OF TRANSLATIONAL MEDICINE 22.1(2024).