Engineering of novel hemagglutinin biosensors for rapid detection and drug screening of Influenza A H7N9 virus

Chen, Xin1; Huang, Yuan1; Gao, Peixuan1; Wu, Fang1; Han, Yongyue1; Zhang, Chuwen1; Hu, Zhuowen3; Zhao, Fang7,8; Shcherbakov, Dmitry N.5,6; Pan, Weiqi4; Niu, Xuefeng4; Li, Xiaoyan3; Liu, Shuwen1,2; Xu, Wei1,9

2024-02-01

10.1016/j.ijbiomac.2023.129126

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES   影响因子和分区

0141-8130

1879-0003

New pathogenic influenza virus strains are constantly emerging, posing a serious risk to both human health and economic growth. To effectively control the spread of this virus, there is an urgent need for early, rapid, sensitive, simple, and cost-effective detection technologies, as well as new and effective antiviral drugs. In this study, we have successfully achieved a significant milestone by successfully fusing the H7N9 influenza virus hemagglutinin (HA) protein with the nano-luciferase component, resulting in the development of a novel set of biosensors. This remarkable achievement marks the first instance of utilizing this biosensor technology for influenza antibody detection. Our biosensor technology also has the potential to facilitate the development of antiviral drugs targeting specific epitopes of the HA protein, providing a promising avenue for the treatment of H7N9 influenza virus infections. Furthermore, our biosensors have broad applications beyond H7N9 influenza virus detection, as they can be expanded for the detection of other pathogens and drug screening applications in the future. By providing a novel and effective solution to the detection and treatment of influenza viruses, our biosensors have the potential to revolutionize the field of infectious disease control.

Hemagglutinin Biosensor Antibody Antiviral drugs

SCI ; EI

英语

其他

National Natural Science Foundation of China["82173865","32041005","82130101"] ; Major Scientific and Technological Projects of Guangdong Province[2019B020202002] ; Chinese Academy of Traditional Chinese Medicine[ZZ13-035-02] ; Guangzhou Science and Technology Program Key Projects[201904020037] ; Open Project of State Key Laboratory of Respiratory Disease[SKLRD-OP-202322] ; Shenzhen Bay Laboratory Open Fund[SZBL2021080601010]

Biochemistry & Molecular Biology ; Chemistry ; Polymer Science

Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science

WOS:001152561500001

ELSEVIER

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Southern Med Univ, Sch Pharmaceut Sci, Guangdong Prov Key Lab New Drug Screening, Guangzhou 510515, Guangdong, Peoples R China
2.Southern Med Univ, Guangdong Prov Inst Nephrol, State Key Lab Organ Failure Res, Guangzhou 510515, Peoples R China
3.Southern Med Univ, Affiliated Hosp 5, Guangzhou 510070, Guangdong, Peoples R China
4.Guangzhou Med Univ, Guangzhou Inst Resp Hlth, Natl Clin Res Ctr Resp Dis, State Key Lab Resp Dis,Affiliated Hosp 1, Guangzhou 510120, Peoples R China
5.State Res Ctr Virol & Biotechnol VECTOR, Koltsov 630559, Russia
6.Altai State Univ, Dept Phys Chem Biol & Biotechnol, Barnaul 656049, Russia
7.Southern Univ Sci & Technol, Peoples Hosp Shenzhen 3, Natl Clin Res Ctr Infect Dis, Shenzhen 518112, Guangdong, Peoples R China
8.Southern Univ Sci & Technol, Affiliated Hosp 2, Shenzhen 518112, Guangdong, Peoples R China
9.Southern Med Univ, Key Lab Infect Dis Res South China, Minist Educ, Guangzhou 510515, Peoples R China

Chen, Xin,Huang, Yuan,Gao, Peixuan,et al. Engineering of novel hemagglutinin biosensors for rapid detection and drug screening of Influenza A H7N9 virus[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,258.

Chen, Xin.,Huang, Yuan.,Gao, Peixuan.,Wu, Fang.,Han, Yongyue.,...&Xu, Wei.(2024).Engineering of novel hemagglutinin biosensors for rapid detection and drug screening of Influenza A H7N9 virus.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,258.

Chen, Xin,et al."Engineering of novel hemagglutinin biosensors for rapid detection and drug screening of Influenza A H7N9 virus".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 258(2024).