Chemical proteomic profiling with photoaffinity labeling strategy identifies antimalarial targets of artemisinin

Gao, Peng1,2; Chen, Jiayun1,2; Sun, Peng1,2; Wang, Jianyou3; Tang, Huan1,2; Xia, Fei1,2; Gu, Liwei1,2; Zhang, Huimin4; Wang, Chen1,2; Wong, Yin Kwan1,2; Zhu, Yinhua1,2; Xu, Chengchao1,2; Wang, Jigang1,2,4,5

2023-12-01

10.1016/j.cclet.2023.108296

CHINESE CHEMICAL LETTERS   影响因子和分区

1001-8417

1878-5964

Present research on the antimalarial mechanisms of artemisinin (ART) is mainly focused on covalent drug binding targets alkylated by free radicals, while non-covalent binding targets have rarely been reported. Here, we developed a novel photoaffinity probe of ART to globally capture and identify the antimalarial target proteins of ART through chemical proteomics. The results demonstrated that ART can bind to par-asite proteins by both covalent and non-covalent modification, and these may jointly contribute to the antimalarial effects. Our work enriches the research on the antimalarial targets of ART, and provides a new perspective for further exploring the antimalarial mechanism of ART. (c) 2023 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.

Artemisinin Antimalarial Chemical proteomic Target identification Photoaffinity probe

SCI

英语

通讯

Establishment of Sino-Austria[2020YFE0205100] ; Fundamental Research Funds for the Central Public Welfare Research Institutes["ZZ14-YQ-050","ZZ14-YQ-051","ZZ14-YQ-052","ZZ14-FL-002","ZZ14-ND-010","ZZ15-ND-10","202228029"] ; Shenzhen Governmental Sustainable Development Fund[KCXFZ20201221173612034] ; Shenzhen Key Laboratory of Kidney Diseases[ZDSYS201504301616234] ; Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties[SZGSP001]

Chemistry

Chemistry, Multidisciplinary

WOS:001148370900001

ELSEVIER SCIENCE INC

CHEMISTRY

Web of Science

1.China Acad Chinese Med Sci, Artemisinin Res Ctr, Beijing 100700, Peoples R China
2.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China
3.Henan Univ, Pharmaceut Coll, Kaifeng 475004, Peoples R China
4.Shandong Acad Chinese Med, Jinan 250014, Peoples R China
5.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Dept Nephrol,Shenzhen Key Lab Kidney Dis,Shenzhen, Shenzhen 518020, Peoples R China

Gao, Peng,Chen, Jiayun,Sun, Peng,et al. Chemical proteomic profiling with photoaffinity labeling strategy identifies antimalarial targets of artemisinin[J]. CHINESE CHEMICAL LETTERS,2023,34(12).

Gao, Peng.,Chen, Jiayun.,Sun, Peng.,Wang, Jianyou.,Tang, Huan.,...&Wang, Jigang.(2023).Chemical proteomic profiling with photoaffinity labeling strategy identifies antimalarial targets of artemisinin.CHINESE CHEMICAL LETTERS,34(12).

Gao, Peng,et al."Chemical proteomic profiling with photoaffinity labeling strategy identifies antimalarial targets of artemisinin".CHINESE CHEMICAL LETTERS 34.12(2023).