The E3 ligase TRIM22 restricts SARS-CoV-2 replication by promoting proteasomal degradation of NSP8

Fan, Lujie1; Zhou, Yuzheng2; Wei, Xiafei2; Feng, Wei2; Guo, Huimin2; Li, Yunfei2; Gao, Xiang2; Zhou, Jian2; Wen, Yanling2; Wu, Yezi2; Shen, Xiaotong2; Liu, Lei1,2; Xu, Gang3; Zhang, Zheng2,4,5

2024-02-14

10.1128/mbio.02320-23

MBIO   影响因子和分区

2150-7511

["Replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome is mediated by a complex of non-structural proteins (NSPs), of which NSP7 and NSP8 serve as subunits and play a key role in promoting the activity of RNA-dependent RNA polymerase (RdRp) of NSP12. However, the stability of subunits of the RdRp complex has rarely been reported. Here, we found that NSP8 was degraded by the proteasome in host cells, and identified tripartite motif containing 22 (TRIM22) as its E3 ligase. The interferon (IFN) signaling pathway was activated upon viral invasion into host cells, and TRIM22 expression increased. TRIM22 interacted with NSP8 and ubiquitinated it at Lys97 via K48-type ubiquitination. TRIM22 overexpression significantly reduced viral RNA and protein levels. Knockdown of TRIM22 enhanced viral replication. This study provides a new explanation for treating patients suffering from SARS-CoV-2 with IFNs and new possibilities for drug development targeting the interaction between NSP8 and TRIM22.IMPORTANCENon-structural proteins (NSPs) play a crucial role in the replication of severe acute respiratory syndrome coronavirus 2, facilitating virus amplification and propagation. In this study, we conducted a comprehensive investigation into the stability of all subunits comprising the RNA-dependent RNA polymerase complex. Notably, our results reveal for the first time that NSP8 is a relatively unstable protein, which is found to be readily recognized and degraded by the proteasome. This degradation process is mediated by the host E3 ligase tripartite motif containing 22 (TRIM22), which is also a member of the interferon stimulated gene (ISG) family. Our study elucidates a novel mechanism of antiviral effect of TRIM22, which utilizes its own E3 ubiquitin ligase activity to hinder viral replication by inducing ubiquitination and subsequent degradation of NSP8. These findings provide new ideas for the development of novel therapeutic strategies. In addition, the conserved property of NSP8 raises the possibility of developing broad antiviral drugs targeting the TRIM22-NSP8 interaction.","Non-structural proteins (NSPs) play a crucial role in the replication of severe acute respiratory syndrome coronavirus 2, facilitating virus amplification and propagation. In this study, we conducted a comprehensive investigation into the stability of all subunits comprising the RNA-dependent RNA polymerase complex. Notably, our results reveal for the first time that NSP8 is a relatively unstable protein, which is found to be readily recognized and degraded by the proteasome. This degradation process is mediated by the host E3 ligase tripartite motif containing 22 (TRIM22), which is also a member of the interferon stimulated gene (ISG) family. Our study elucidates a novel mechanism of antiviral effect of TRIM22, which utilizes its own E3 ubiquitin ligase activity to hinder viral replication by inducing ubiquitination and subsequent degradation of NSP8. These findings provide new ideas for the development of novel therapeutic strategies. In addition, the conserved property of NSP8 raises the possibility of developing broad antiviral drugs targeting the TRIM22-NSP8 interaction."]

SARS-CoV-2 NSP8 TRIM22 ubiquitination protein degradation

SCI

英语

通讯

National Key Research and Development Program of China["2021YFA0910900","2023YFC2606300"] ; National Natural Science Foundation of China[92369109] ; Guangdong Science and Technology Plan Project, Construction of high-level biosafety laboratories[2021B1212030010] ; Guangdong Basic and Applied Basic Research Foundation[2021A1515111218] ; Shenzhen Science and Technology Program[JCYJ20220818103017036] ; Natural Science Research Project of Anhui Educational Committee[2022AH030080]

Microbiology

Microbiology

WOS:001148807000001

AMER SOC MICROBIOLOGY

Web of Science

1.Guangzhou Med Univ, Guangzhou Lab, Guangzhou, Peoples R China
2.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Affiliated Hosp 2, Inst Hepatol,Sch Med,Natl Clin Res Ctr Infect Dis, Shenzhen, Guangdong Provi, Peoples R China
3.Anhui Med Univ, Sch Basic Med Sci, Dept Microbiol, Hefei, Peoples R China
4.Guangdong Key Lab Antiinfect Drug Qual Evaluat, Shenzhen, Guangdong, Peoples R China
5.Chinese Acad Med Sci, Shenzhen Res Ctr Communicable Dis Diag Treatment, Shenzhen, Guangdong, Peoples R China

Fan, Lujie,Zhou, Yuzheng,Wei, Xiafei,et al. The E3 ligase TRIM22 restricts SARS-CoV-2 replication by promoting proteasomal degradation of NSP8[J]. MBIO,2024,15(2).

Fan, Lujie.,Zhou, Yuzheng.,Wei, Xiafei.,Feng, Wei.,Guo, Huimin.,...&Zhang, Zheng.(2024).The E3 ligase TRIM22 restricts SARS-CoV-2 replication by promoting proteasomal degradation of NSP8.MBIO,15(2).

Fan, Lujie,et al."The E3 ligase TRIM22 restricts SARS-CoV-2 replication by promoting proteasomal degradation of NSP8".MBIO 15.2(2024).